| Calcium carbonate microparticles used as a gene vector for delivering p53 gene into cancer cells. | |
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MedLine Citation:
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PMID: 22529011 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Calcium carbonate (CaCO(3) ) microparticles were for the first time used for efficient delivery of p53 gene to transfect human cancer cells HeLa. CaCO(3) microparticles (2-4 μm) absorbed pEGFP-C1-p53 (expressing GFP-P53 fusion protein) to transfect HeLa cells. Flow cytometer (FCM) was used to evaluate the gene transfection efficiency in HeLa cells, which were stably transduced with a green fluorescent protein gene. In this study, CaCO(3) delivering pEGFP-C1-p53 could transfect about 5% of the tumor cells in culture. However, the efficiency of tumor cell apoptosis was surprisingly up to 80%. Meanwhile, the results of MTT assay and crystal violet staining showed that the CaCO(3) microparticles had low cytotoxicity. These findings showed that CaCO(3) microparticles were perspective to be used as new vectors for gene therapy. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012. |
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Authors:
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Xiangdong Kong; Suju Xu; Xiumei Wang; Fuzhai Cui; Juming Yao |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-4-24 |
Journal Detail:
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Title: Journal of biomedical materials research. Part A Volume: - ISSN: 1552-4965 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-4-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101234237 Medline TA: J Biomed Mater Res A Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
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Xinyuan Institute of Medicine and Biotechnology, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China; The Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, College of Materials and Textile, Zhejiang Sci-Tech University, Hangzhou 310018, China. kongxiangdong@gmail.com. |
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