Document Detail


Calcium antagonists and heat-induced hepatic injury.
MedLine Citation:
PMID:  2890236     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several laboratories have demonstrated the value of the isolated perfused rat liver as a suitable model for heat-induced hepatic injury in vivo. Membrane changes caused by perfusion of rat livers at 42 degrees C for 90 min were similar to those induced by toxic chemicals or hypoxia. In an evaluation of several categories of drugs reported to reduce cell injury, calcium antagonists (nifedipine, dantrolene, and verapamil), were evaluated for their therapeutic potential for heat injury. Isolated rat livers were perfused at 42 degrees C for 90 min with and without calcium antagonists. Livers were also perfused at 37 degrees C. Potassium and transaminase leakage, bile production and ultrastructure were used to evaluate their responses. Neither of the three calcium antagonists significantly improved any of the functional parameters measured. However, dantrolene produced dilated or vesicular rough endoplasmic reticulum in the heated livers. These changes suggest selective intracellular action on endoplasmic reticulum of heated livers. Ring-shaped mitochondria and vesicular endoplasmic reticulum were observed in the heated, verapamil-treated livers, but these could not be quantitatively distinguished from controls. Nifedipine did not appear to alter intracellular membranes, but did increase bile production.
Authors:
W Bowers; P Daum; M Blaha; C Stevens; I Castro; I Leav
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Virchows Archiv. B, Cell pathology including molecular pathology     Volume:  53     ISSN:  0340-6075     ISO Abbreviation:  Virchows Arch., B, Cell Pathol.     Publication Date:  1987  
Date Detail:
Created Date:  1987-12-11     Completed Date:  1987-12-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9316922     Medline TA:  Virchows Arch B Cell Pathol Incl Mol Pathol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  235-42     Citation Subset:  IM    
Affiliation:
U.S. Army Research Institute of Environmental Medicine, Natick, Massachusetts 01760-5007.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aspartate Aminotransferases / analysis
Bile / secretion
Calcium Channel Blockers / pharmacology*
Dantrolene / pharmacology
Hyperthermia, Induced / adverse effects*
Liver / drug effects*,  ultrastructure
Male
Microscopy, Electron
Nifedipine / pharmacology
Perfusion
Rats
Verapamil / pharmacology
Chemical
Reg. No./Substance:
0/Calcium Channel Blockers; 21829-25-4/Nifedipine; 52-53-9/Verapamil; 7261-97-4/Dantrolene; EC 2.6.1.1/Aspartate Aminotransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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