| Calcium-activated, phospholipid-dependent protein kinase in pancreatic acinar cells. | |
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MedLine Citation:
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PMID: 3159267 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the present study we partially purified calcium-activated, phospholipid-dependent protein kinase (protein kinase C) from pancreatic acinar cells of the guinea pig using diethylaminoethylcellulose and Sephadex G-150 chromatography and characterized the dependence of the enzyme on calcium, phospholipids, diacylglycerol (diolein), and the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA). The enriched preparation of protein kinase C contained no cyclic nucleotide-dependent or calcium-dependent, calmodulin-dependent protein kinase activity. The values of Km for H1-histone and ATP were 0.74 +/- 0.22 and 13.1 +/- 3.2 microM, respectively. Pancreatic protein kinase C demonstrated an absolute requirement for calcium and phospholipid for its activation, and diolein or TPA increased the affinity of the enzyme for calcium by 10-fold. With phosphatidylserine the calcium concentration that caused a half-maximal activation (Ka) was 74 +/- 17 microM, whereas with phosphatidylserine and diolein or TPA the Ka for calcium was 7.9 +/- 1.6 or 6.8 +/- 1.3 microM, respectively. Adding phosphatidylethanolamine and phosphatidylserine decreased the Ka for calcium to 2.0 +/- 0.9 microM with diolein and to 0.7 +/- 0.4 microM with TPA. Activation of protein kinase C by TPA and diolein was identical with calcium concentrations greater than 1 microM, but at low calcium concentrations (less than 1 microM) in the presence of phospholipids, maximally effective concentrations of diolein caused only 55% of the activation seen with TPA. In addition to TPA, other phorbol esters such as phorbol dibutyrate and phorbol diacetate, but not phorbol itself, activated protein kinase C.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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M Noguchi; H Adachi; J D Gardner; R T Jensen |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: The American journal of physiology Volume: 248 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1985 Jun |
Date Detail:
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Created Date: 1985-07-24 Completed Date: 1985-07-24 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: G692-701 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium / metabolism Chromatography, DEAE-Cellulose Chromatography, Gel Diglycerides / metabolism Enzyme Activation / drug effects Guinea Pigs Islets of Langerhans / enzymology* Male Phorbol Esters / pharmacology Phospholipids / metabolism Protein Kinase C Protein Kinases / isolation & purification, metabolism* Stimulation, Chemical |
| Chemical | |
Reg. No./Substance:
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0/Diglycerides; 0/Phorbol Esters; 0/Phospholipids; 7440-70-2/Calcium; EC 2.7.-/Protein Kinases; EC 2.7.11.13/Protein Kinase C |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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