Document Detail


Calcium sensitivity for hypoxia in PGNs with PC-12 cells in co-culture.
MedLine Citation:
PMID:  19536473     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Calcium sensitivity of petrosal ganglion neurons (PGNs) to chemical stimuli with and without PC-12 cells in co-culture instead of glomus is not known- the idea being that two types of unusual cells could form synapse and provide a model for studies of chemotransduction. Calcium levels in the PGNs were measured in the presence of different chemical stimuli in the bath medium. Remarkably, the PGNs alone were not sensitive to hypoxia (10 torr), PCO ( approximately 300 torr in normoxa) nor to ATP (100microM) but they developed the sensitivity to these stimuli in synaptic contact with PC-12 cells. The sharp rise in calcium level was suppressed (2/3) by suramin (100microM), a purinergic blocker, and the remaining 1/3 was blocked by hexomethonium, a cholinergic blocker. Taken together, these observations suggest that PGNs developed neurotransmission when in contact with PC-12 cells, as if the latter substituting for glomus cells, thus providing a model for chemotransduction studies. The reason for the insensitivity of PGNs alone to the chemical stimuli is unknown at this time.
Authors:
G P Patel; S M Baby; A Roy; S Lahiri
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Advances in experimental medicine and biology     Volume:  648     ISSN:  0065-2598     ISO Abbreviation:  Adv. Exp. Med. Biol.     Publication Date:  2009  
Date Detail:
Created Date:  2009-06-18     Completed Date:  2009-07-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0121103     Medline TA:  Adv Exp Med Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  121-4     Citation Subset:  IM    
Affiliation:
University of Pennsylvania Medical Center, Department of Physiology, 19104, Philadelphia, PA, USA.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / pharmacology
Animals
Calcium / metabolism*
Carbon Monoxide / pharmacology
Carotid Body / cytology,  drug effects,  metabolism
Cell Hypoxia
Coculture Techniques
Ganglia, Sensory / cytology*
Intracellular Space / drug effects,  metabolism
Neurons / cytology,  drug effects*,  metabolism*
PC12 Cells
Rats
Chemical
Reg. No./Substance:
56-65-5/Adenosine Triphosphate; 630-08-0/Carbon Monoxide; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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