| Calcium phosphate cement with BMP-2-loaded gelatin microspheres enhances bone healing in osteoporosis: a pilot study. | |
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MedLine Citation:
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PMID: 20306162 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The capacity for bone healing reportedly is limited in osteoporosis with a less than ideal environment for healing of bone grafts. We therefore developed a composite bone substitute with rhBMP-2 loaded gelatin microsphere (GM) and calcium phosphate cement (CPC) to use in osteoporosis. QUESTIONS/PURPOSES: We asked whether (1) controlled release of rhBMP-2 could be improved in this composite bone substitute and (2) increasing factors released from the bone substitute could accelerate osteoporotic bone healing. METHODS: We soaked rhBMP-2/GM/CPC and rhBMP-2/CPC composites in simulated body fluid for 28 days and then determined the amount of rhBMP-2 released. Both composites were implanted in bone defects of osteoporotic goats and left in place for 45 and 140 days; the specimens then were evaluated mechanically (pushout test) and morphologically (CT scanning, histology). RESULTS: The in vitro study showed the new composite released more rhBMP-2 compared with rhBMP-2/CPC. CT showed the defects healed more quickly with new grafts. The bone mineralization rate was greater in rhBMP-2/GM/CPC than in rhBMP-2/CPC after 45 days of implantation and the pushout test was stronger after 45 and 140 days of implantation. CONCLUSIONS: The new graft composite released more loaded factors and appeared to repair osteoporotic bone defects. CLINICAL RELEVANCE: These preliminary data suggest the new composite can be used as a bone substitute to accelerate healing of fractures and bone defects in osteoporosis. |
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Authors:
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Meng Li; Xingyan Liu; Xudong Liu; Baofeng Ge |
Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-20 |
Journal Detail:
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Title: Clinical orthopaedics and related research Volume: 468 ISSN: 1528-1132 ISO Abbreviation: Clin. Orthop. Relat. Res. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-06-09 Completed Date: 2010-06-28 Revised Date: 2011-08-01 |
Medline Journal Info:
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Nlm Unique ID: 0075674 Medline TA: Clin Orthop Relat Res Country: United States |
Other Details:
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Languages: eng Pagination: 1978-85 Citation Subset: AIM; IM |
Affiliation:
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Orthopedic Trauma Institute of CPLA, Lanzhou General Hospital of CPLA, 333 Bing-he-nan Road, Lanzhou, 730050, Gansu Province, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bone Cements Bone Morphogenetic Protein 2 / administration & dosage*, chemistry Bone Substitutes / administration & dosage*, chemistry Calcium Phosphates / administration & dosage*, chemistry Cementation Compressive Strength / drug effects Disease Models, Animal Drug Carriers Female Goats / physiology* Lumbar Vertebrae / drug effects, surgery Microspheres Osteogenesis / drug effects* Pilot Projects Recombinant Proteins / administration & dosage*, chemistry Stress, Mechanical Wound Healing / drug effects |
| Chemical | |
Reg. No./Substance:
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0/BMP2 protein, human; 0/Bone Cements; 0/Bone Morphogenetic Protein 2; 0/Bone Substitutes; 0/Calcium Phosphates; 0/Drug Carriers; 0/Recombinant Proteins |
| Comments/Corrections | |
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