Document Detail

Calcitriol treatment improves methyl aminolaevulinate-based photodynamic therapy in human squamous cell carcinoma A431 cells.
MedLine Citation:
PMID:  19438460     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Photodynamic therapy (PDT) using methyl aminolaevulinate (MAL) provides a new, approved method for treatment of skin cancer and its precursors. However, MAL-based PDT is not very efficient for poorly differentiated skin carcinoma. Thus, novel strategies to enhance the PDT effect are needed. OBJECTIVES: In order to improve the efficacy of MAL-based PDT, we investigated the effect of adding calcitriol, a prodifferentiation hormone, to human squamous cell carcinoma A431 cells in vitro. METHODS: A short course (24 h) of calcitriol pretreatment was applied in A431 cells, and, subsequently, MAL-induced protoporphyrin IX (PpIX) was measured. RESULTS: Calcitriol pretreatment of the cells elevated their PpIX levels. Furthermore, the cell damage after exposure to blue light was significantly higher in calcitriol-treated cells. Increased photoinactivation correlated with higher levels of PpIX in the calcitriol-pretreated cells. CONCLUSIONS: Calcitriol enhances MAL-based PDT in A431 cells.
E Cicarma; M Tuorkey; A Juzeniene; L-W Ma; J Moan
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-29
Journal Detail:
Title:  The British journal of dermatology     Volume:  161     ISSN:  1365-2133     ISO Abbreviation:  Br. J. Dermatol.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-08-11     Completed Date:  2009-12-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0004041     Medline TA:  Br J Dermatol     Country:  England    
Other Details:
Languages:  eng     Pagination:  413-8     Citation Subset:  IM    
Department of Radiation Biology, The Norwegian Radium Hospital, Rikshospitalet University Hospital, Montebello, Oslo 0310, Norway.
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MeSH Terms
Aminolevulinic Acid / analogs & derivatives*,  metabolism,  therapeutic use
Calcitriol / pharmacology*
Carcinoma, Basal Cell / drug therapy*,  metabolism
Carcinoma, Squamous Cell / drug therapy*,  metabolism
Cell Line, Tumor
Photochemotherapy / methods*
Photosensitizing Agents / metabolism*,  therapeutic use
Protoporphyrins / biosynthesis*
Tumor Stem Cell Assay
Reg. No./Substance:
0/Photosensitizing Agents; 0/Protoporphyrins; 0/methyl 5-aminolevulinate; 106-60-5/Aminolevulinic Acid; 32222-06-3/Calcitriol; 553-12-8/protoporphyrin IX

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