| Calcitonin gene-related peptide suppresses isoprenaline-induced cardiomyocyte apoptosis through regulation of microRNA-1 and microRNA-133a expression. | |
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MedLine Citation:
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PMID: 22086007 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Objective To explore the inhibitory effect of calcitonin gene-related peptide (CGRP) on cardiomyocyte apoptosis and the underlying mechanism. Methods In cultured rat cardiomyocytes,apoptosis was induced by the incubation of isoprenaline (10(-5) mol/L) for 48 h. CGRP (10(-8) or 10(-7) mol/L) was administrated for 1 h before incubating isoprenaline to evaluate its effect on cardiomyocyte apoptosis. At the end of the drug treatment,the rate of apoptotic cells and intracellular reactive oxygen species (ROS) were determined,and RNA was extracted to examine the expression of microRNA-1 and microRNA-133a. Results Isoprenaline significantly increased the rate of apoptotic cells and intracellular ROS production concomitantly with the increased microRNA-1 expression and the decreased microRNA-133a expression,which were inhibited by pretreatment with CGRP. The effects of CGRP were reversed by CGRP receptor antagonist. Conclusion CGRP can inhibit the isoprenaline-induced cardiomyocyte apoptosis. The beneficial effect of CGRP is related to regulating microRNA-1 and microRNA-133a expression through the prevention of isoprenaline-induced ROS production. |
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Authors:
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Jianzhe Li; Jun Peng; Chenjing Wang; Hanwu Deng; Yuanjian Li |
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Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences Volume: 36 ISSN: 1672-7347 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-11-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101230586 Medline TA: Zhong Nan Da Xue Xue Bao Yi Xue Ban Country: - |
Other Details:
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Languages: ENG Pagination: 964-971 Citation Subset: - |
Affiliation:
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Department of Pharmacology,School of Pharmaceutical Sciences,Central South University,Changsha 410078; Department of Pharmacy,Ruikang Hospital,Guangxi College of Traditional Chinese Medicine,Nanning 530011,China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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