Document Detail

Calcitonin gene-related peptide stimulates human villous trophoblast cell differentiation in vitro.
MedLine Citation:
PMID:  16772432     Owner:  NLM     Status:  MEDLINE    
Calcitonin gene-related peptide (CGRP) is a multifunctional peptide present in both maternal and fetal circulations in pregnancy. Its receptors have been identified on human trophoblast cells, but the role of CGRP in trophoblast differentiation remains unknown. This study was designed to determine the effect of CGRP on the differentiation of villous trophoblasts isolated from normal human term placentae. The morphological and functional differentiation of the trophoblast cells were assessed by desmosomal protein immunofluorescent staining and the quantification of hCG, estrogen and progesterone secretion. Results showed that (i) exposure of villous trophoblast cells to CGRP led to a dose-dependent increase in intracellular cyclic adenosine monophosphate (cAMP) accumulation; (ii) immunofluorescent staining with antidesmosomal antibody was identified at the boundaries between aggregated cytotrophoblast cells, and these stainings disappeared when cells fused to form syncytiotrophoblast cells; (iii) the formation of multinucleated syncytiums in primary cultured cytotrophoblasts was stimulated by CGRP as evidenced by the changes in antidesmosomal staining; (iv) CGRP increases trophoblast hCG secretion in a time- and dose-dependent manner, and this secretion was blocked by CGRP antagonist, CGRP(8-37), and (v) both 17beta-estradiol (E(2)) and progesterone concentrations in the culture medium were increased by CGRP, and these increases were dose dependent. These observations suggest that CGRP may be involved in the morphological and functional differentiation of trophoblast cells, and these actions might be attributed to CGRP-induced intracellular cAMP accumulation.
K E Green; C Thota; G D V Hankins; C Yallampalli; Yuan-Lin Dong
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2006-06-13
Journal Detail:
Title:  Molecular human reproduction     Volume:  12     ISSN:  1360-9947     ISO Abbreviation:  Mol. Hum. Reprod.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-07-14     Completed Date:  2006-10-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9513710     Medline TA:  Mol Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  443-50     Citation Subset:  IM    
Department of Obstetrics and Gynecology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555-1062, USA.
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MeSH Terms
Calcitonin Gene-Related Peptide / pharmacology*
Cell Differentiation / drug effects*
Cell Survival / drug effects
Cells, Cultured
Cyclic AMP / metabolism
Dose-Response Relationship, Drug
Fluorescent Antibody Technique
Giant Cells / metabolism
Keratins / metabolism
Placenta / cytology,  metabolism
Pregnancy Proteins / metabolism
Receptors, Calcitonin Gene-Related Peptide / chemistry,  metabolism
Time Factors
Trophoblasts / cytology,  drug effects*,  metabolism
Grant Support
Reg. No./Substance:
0/Pregnancy Proteins; 0/Receptors, Calcitonin Gene-Related Peptide; 60-92-4/Cyclic AMP; 68238-35-7/Keratins; 83652-28-2/Calcitonin Gene-Related Peptide

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