| Calcitonin gene-related peptide enhances the rate of desensitization of the nicotinic acetylcholine receptor in cultured mouse muscle cells. | |
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MedLine Citation:
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PMID: 2456580 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Calcitonin gene-related peptide (CGRP) is a neuropeptide that coexists with acetylcholine in spinal cord motoneurons. The effects of CGRP on the functional properties of the nicotinic acetylcholine receptor (AcChoR) were examined by electrophysiological methods. Using the whole-cell patch-clamp technique and a mouse cell line derived from soleus muscle, we found that CGRP produces a progressive and reversible enhancement of the rapid-decay phase of AcChoR desensitization. Single-channel data further show that CGRP decreases acetylcholine-activated channel opening frequency. This decrease occurs when CGRP and acetylcholine are applied on different cell-surface areas and thus is likely mediated by a second-messenger system. CGRP is also shown to increase cAMP accumulation in this cell line. The effects of CGRP on macroscopic acetylcholine-activated currents are mimicked by external application of forskolin (10 microM) or by internal perfusion of the cell with cAMP (1 microM). In both these cases, further application of CGRP produces no additional enhancement of AcChoR desensitization. These results suggest that, on mouse muscle cells, CGRP regulates AcChoR desensitization by a mechanism that involves, at least in part, cAMP-dependent phosphorylation of the AcChoR. |
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Authors:
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C Mulle; P Benoit; C Pinset; M Roa; J P Changeux |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 85 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1988 Aug |
Date Detail:
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Created Date: 1988-09-07 Completed Date: 1988-09-07 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 5728-32 Citation Subset: IM |
Affiliation:
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Unité de Neurobiologie Moléculaire, Centre National de la Recherche Scientifique, Paris, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcitonin / genetics Calcitonin Gene-Related Peptide Calcium / metabolism Clone Cells Cyclic AMP / metabolism Electrophysiology Forskolin / pharmacology Ion Channels / drug effects* Mice Mice, Inbred C3H Muscles / cytology, metabolism* Neuropeptides / pharmacology* Phosphorylation Receptors, Cholinergic / drug effects*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Ion Channels; 0/Neuropeptides; 0/Receptors, Cholinergic; 60-92-4/Cyclic AMP; 66428-89-5/Forskolin; 7440-70-2/Calcium; 83652-28-2/Calcitonin Gene-Related Peptide; 9007-12-9/Calcitonin |
| Comments/Corrections | |
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