| Calcineurin immunoreactivity in Alzheimer's disease. | |
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MedLine Citation:
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PMID: 7925818 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Aberrant phosphorylation of tau is linked to formation of the paired helical filaments (PHF) seen in Alzheimer's disease. Protein kinases such as mitogen-activated protein kinase, and calcium-regulated protein kinases may, in part, be responsible for addition of phosphate groups to serine residues of PHFtau; however, less is known concerning the phosphatases which regulate tau. In this report, we used several well-characterized antibodies to document calcineurin immunoreactivity in brain tissue from patients with Alzheimer's disease. We now report that levels of immunoreactive calcineurin are not significantly altered in neocortex and cerebellum of Alzheimer's patients relative to similar regions of age-matched controls. Immunocytochemical studies indicated that calcineurin immunoreactivity was present in dendrites and perikarya of many different neuronal populations in both control and Alzheimer brain. When specific antibodies against PHFtau were used in double-labeling experiments with anti-calcineurin antibodies, calcineurin immunoreactivity was seen in association with neurofibrillary tangles. However, calcineurin was not seen in all tangle bearing neurons. These data suggest that calcineurin levels per se are not significantly altered in Alzheimer's disease, but that calcineurin is distributed around some neurofibrillary tangles and may play a role in regulation of tau phosphorylation. |
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Authors:
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M L Billingsley; C Ellis; R L Kincaid; J Martin; M L Schmidt; V M Lee; J Q Trojanowski |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Experimental neurology Volume: 126 ISSN: 0014-4886 ISO Abbreviation: Exp. Neurol. Publication Date: 1994 Apr |
Date Detail:
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Created Date: 1994-11-17 Completed Date: 1994-11-17 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0370712 Medline TA: Exp Neurol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 178-84 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, Pennsylvania State University College of Medicine, Hershey 17033. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aged Aged, 80 and over Alzheimer Disease / metabolism*, pathology* Calcineurin Calmodulin-Binding Proteins / analysis* Cerebellum / metabolism, pathology Humans Immunohistochemistry Occipital Lobe / metabolism, pathology Organ Specificity Phosphoprotein Phosphatases / analysis* Reference Values tau Proteins / analysis |
| Grant Support | |
ID/Acronym/Agency:
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AG-09215/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Calmodulin-Binding Proteins; 0/tau Proteins; EC 3.1.3.16/Calcineurin; EC 3.1.3.16/Phosphoprotein Phosphatases |
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