Document Detail

Calbindin-D28K in the nucleus of nerve growth factor-treated PC12 cells.
MedLine Citation:
PMID:  8981395     Owner:  NLM     Status:  MEDLINE    
Calbindin-D28K (CB) is a calcium-binding protein found within a subset of neurons, which has been proposed to buffer intracellular calcium and to protect cells from calcium-induced neurodegeneration. The rat pheochromocytoma PC12 cell line has been an invaluable tool for studying neuronal development and characterizing the molecular actions of nerve growth factor (NGF). Using Western and RNAse protection analyses and immunocytochemical and cell fractionation techniques, we now report that NGF induces the expression of CB not only in the cytoplasm and neurites, but also within the nuclei of PC12 cells. These data indicate that PC12 cells can serve as a useful model system in which to study the functions of CB and the role of nuclear CB.
A McMahon; E D Lephart; S Chi; M Lee; A M Iacopino; D C German
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neuroreport     Volume:  7     ISSN:  0959-4965     ISO Abbreviation:  Neuroreport     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1997-04-01     Completed Date:  1997-04-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9100935     Medline TA:  Neuroreport     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2419-22     Citation Subset:  IM    
Department of Psychiatry, University of Texas Southwestern Medical Center, Dallas 75235-9070, USA.
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MeSH Terms
Calcium-Binding Proteins / metabolism*
Cell Nucleus / drug effects,  metabolism*
Cells, Cultured
Nerve Growth Factors / pharmacology*
PC12 Cells / drug effects,  metabolism*
Grant Support
Reg. No./Substance:
0/Calcium-Binding Proteins; 0/Nerve Growth Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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