| A Cajal body-independent pathway for telomerase trafficking in mice. | |
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MedLine Citation:
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PMID: 20633556 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The intranuclear trafficking of human telomerase involves a dynamic interplay between multiple nuclear sites, most notably Cajal bodies and telomeres. Cajal bodies are proposed to serve as sites of telomerase maturation, storage, and assembly, as well as to function in the cell cycle-regulated delivery of telomerase to telomeres in human cells. Here, we find that telomerase RNA does not localize to Cajal bodies in mouse cells, and instead resides in separate nuclear foci throughout much of the cell cycle. However, as in humans, mouse telomerase RNA (mTR) localizes to subsets of telomeres specifically during S phase. The localization of mTR to telomeres in mouse cells does not require coilin-containing Cajal bodies, as mTR is found at telomeres at similar frequencies in cells from wild-type and coilin knockout mice. At the same time, we find that human TR localizes to Cajal bodies (as well as telomeres) in mouse cells, indicating that the distinct trafficking of mTR is attributable to an intrinsic property of the RNA (rather than a difference in the mouse cell environment such as the properties of mouse Cajal bodies). We also find that during S phase, mTR foci coalesce into short chains, with at least one of the conjoined mTR foci co-localizing with a telomere. These findings point to a novel, Cajal body-independent pathway for telomerase biogenesis and trafficking in mice. |
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Authors:
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Rebecca L Tomlinson; Jian Li; Bradley R Culp; Rebecca M Terns; Michael P Terns |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-07-13 |
Journal Detail:
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Title: Experimental cell research Volume: 316 ISSN: 1090-2422 ISO Abbreviation: Exp. Cell Res. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-24 Completed Date: 2010-11-10 Revised Date: 2011-10-17 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: United States |
Other Details:
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Languages: eng Pagination: 2797-809 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Departments of Biochemistry and Molecular Biology, and Genetics, University of Georgia, Athens, GA 30602, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Binding Sites Cell Cycle Coiled Bodies* Mice Protein Transport RNA / metabolism* S Phase Telomerase / biosynthesis, genetics, metabolism* Telomere / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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GM07103/GM/NIGMS NIH HHS; R01 CA104676/CA/NCI NIH HHS; R01 CA104676-04S2/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/telomerase RNA; 63231-63-0/RNA; EC 2.7.7.49/Telomerase |
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