Document Detail


Caged compounds: photorelease technology for control of cellular chemistry and physiology.
MedLine Citation:
PMID:  17664946     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Caged compounds are light-sensitive probes that functionally encapsulate biomolecules in an inactive form. Irradiation liberates the trapped molecule, permitting targeted perturbation of a biological process. Uncaging technology and fluorescence microscopy are 'optically orthogonal': the former allows control, and the latter, observation of cellular function. Used in conjunction with other technologies (for example, patch clamp and/or genetics), the light beam becomes a uniquely powerful tool to stimulate a selected biological target in space or time. Here I describe important examples of widely used caged compounds, their design features and synthesis, as well as practical details of how to use them with living cells.
Authors:
Graham C R Ellis-Davies
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Nature methods     Volume:  4     ISSN:  1548-7091     ISO Abbreviation:  Nat. Methods     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-07-31     Completed Date:  2007-10-01     Revised Date:  2014-10-24    
Medline Journal Info:
Nlm Unique ID:  101215604     Medline TA:  Nat Methods     Country:  United States    
Other Details:
Languages:  eng     Pagination:  619-28     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Biology*
Microscopy, Fluorescence
Molecular Probes*
Photochemistry
Grant Support
ID/Acronym/Agency:
GM53395/GM/NIGMS NIH HHS; GM65473/GM/NIGMS NIH HHS; MH0717505/MH/NIMH NIH HHS; R01 GM053395/GM/NIGMS NIH HHS; R01 GM053395-12/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Molecular Probes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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