Document Detail

Caffeine-induced apoptosis reveals a persistent lesion after treatment with bromodeoxyuridine and ultraviolet-B light.
MedLine Citation:
PMID:  9189164     Owner:  NLM     Status:  MEDLINE    
Ultraviolet-B (UVB) light treatment of synchronized V79 Chinese hamster cells after pulse-labeling with bromodeoxyuridine (BrdUrd) reveals a marked age response for cell killing. Incubation after treatment in growth medium containing caffeine increases cell killing during the resistant portions of the cell cycle, resulting in a much less marked age response to UVB irradiation. Examination of the split-dose survival curves for BrdUrd and UVB light in the presence or absence of caffeine indicates that sensitization by caffeine is completely independent of the sparing effect of dose fractionation. Further, sensitization by caffeine is nearly complete after the first mitosis after the UVB exposure. With delayed addition of caffeine, however, nearly full sensitization can be elicited as late as two cell cycles after the treatment with BrdUrd and UVB light. Also, synchronized cells exposed to caffeine after treatment with BrdUrd and UVB in the S phase cease to incorporate radiolabeled thymidine and undergo apoptosis after the second mitosis. Thus exposure to caffeine reveals a persistent sensitivity lasting for at least two cell cycles, after the injury induced by treatment with BrdUrd and UVB.
M P Hagan; K L Hopcia; F C Sylvester; K D Held
Related Documents :
2067544 - Expression dynamics of transforming phenotypes in x-irradiated syrian golden hamster em...
16966184 - Induction of radiation resistance by a heat shock protein inhibitor, knk437, in human g...
6897104 - The ability of rat transplantable lymphosarcoma cells to synthesize immunoglobulins.
16544934 - Analysis of the cell cycle of fibroblasts derived from the lec rat after x-irradiation.
10762404 - Cellular basis of wing size variation in drosophila melanogaster: a comparison of latit...
6196084 - Generation of a monoclonal antibody (epenl) which binds selectively to murine ependymal...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Radiation research     Volume:  147     ISSN:  0033-7587     ISO Abbreviation:  Radiat. Res.     Publication Date:  1997 Jun 
Date Detail:
Created Date:  1997-07-03     Completed Date:  1997-07-03     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0401245     Medline TA:  Radiat Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  674-9     Citation Subset:  IM; S    
Department of Radiation Oncology, University of Florida, Shands Cancer Center, Gainesville 32610, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Apoptosis / drug effects*
Bromodeoxyuridine / pharmacology
Caffeine / pharmacology*
Cells, Cultured
DNA Damage*
Ultraviolet Rays
Reg. No./Substance:
58-08-2/Caffeine; 59-14-3/Bromodeoxyuridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Where are the sugar radicals in irradiated DNA?
Next Document:  Stem cell factor has contrasting effects in combination with 5-fluorouracil or total-body irradiatio...