Document Detail


Caffeic acid phenethyl ester triggers apoptosis through induction of loss of mitochondrial membrane potential in CCRF-CEM cells.
MedLine Citation:
PMID:  20221636     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
PURPOSE: CAPE (caffeic acid phenethyl ester) is one of the most valuable and investigated component of propolis which is composed by honeybees. In the current study, we aimed at examining apoptotic effects of CAPE on CCRF-CEM leukemic cells and at determining the roles of mitochondrial membrane potential (MMP) in cell death.
METHODS: Trypan blue and XTT methods were used to evaluate the cytotoxicity. Apoptosis was examined by ELISA-based oligonucleotide and acridine orange/ethidium bromide dye techniques. Loss of mitochondrial membrane potential was evaluated using JC-1 dye by flow cytometric analysis and under fluorescent microscope.
RESULTS: We detected the time- and dose-dependent increases in cytotoxic effect of CAPE on CCRF-CEM cells. ELISA and acridine orange/ethidium bromide results showed that apoptotic cell population increased significantly in CCRF-CEM cells exposed to increasing concentrations of CAPE. On the other hand, there was significant loss of MMP determined in response to CAPE in CCRF-CEM cells.
CONCLUSION: This in vitro data by being supported with clinical data may open the way of the potential use of CAPE for the treatment of leukemia.
Authors:
Cığır Biray Avcı; Cumhur Gündüz; Yusuf Baran; Fahri Sahin; Sunde Yılmaz; Zeynep Ozlem Dogan; Güray Saydam
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Publication Detail:
Type:  Journal Article     Date:  2010-03-10
Journal Detail:
Title:  Journal of cancer research and clinical oncology     Volume:  137     ISSN:  1432-1335     ISO Abbreviation:  J. Cancer Res. Clin. Oncol.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7902060     Medline TA:  J Cancer Res Clin Oncol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  41-7     Citation Subset:  IM    
Affiliation:
Department of Medical Biology, School of Medicine, Ege University, Bornova, Izmir 35100, Turkey.
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