Document Detail


Caenorhabditis elegans lin-25: cellular focus, protein expression and requirement for sur-2 during induction of vulval fates.
MedLine Citation:
PMID:  9806929     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Induction of vulval fates in the C. elegans hermaphrodite is mediated by a signal transduction pathway involving Ras and MAP kinase. Previous genetic analysis has suggested that two potential targets of this pathway in the vulva precursor cells are two novel proteins, LIN-25 and SUR-2. In this report, we describe further studies of lin-25. The results of a genetic mosaic analysis together with those of experiments in which lin-25 was expressed under the control of an heterologous promoter suggest that the major focus of lin-25 during vulva induction is the vulva precursor cells themselves. We have generated antisera to LIN-25 and used these to analyse the pattern of protein expression. LIN-25 is present in all six precursor cells prior to and during vulva induction but later becomes restricted to cells of the vulval lineages. Mutations in genes in the Ras/MAP kinase pathway do not affect the pattern of expression but the accumulation of LIN-25 is reduced in the absence of sur-2. Overexpression of LIN-25 does not rescue sur-2 mutant defects suggesting that LIN-25 and SUR-2 may function together. LIN-25 is also expressed in the lateral hypodermis. Overexpression of LIN-25 disrupts lateral hypodermal cell fusion, suggesting that lin-25 may play a role in regulating cell fusions in C. elegans.
Authors:
L Nilsson; X Li; T Tiensuu; R Auty; I Greenwald; S Tuck
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  125     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-03-18     Completed Date:  1999-03-18     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  4809-19     Citation Subset:  IM    
Affiliation:
Umeå Center for Molecular Pathogenesis, Umeå University, S-901 87 Umeå, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Animals
Caenorhabditis elegans / embryology*,  genetics*
Caenorhabditis elegans Proteins*
DNA-Binding Proteins / genetics*,  physiology
Embryonic Induction
Female
Fluorescent Antibody Technique, Indirect
Gene Expression Regulation, Developmental*
Genotype
Helminth Proteins / genetics*
Hermaphroditism
Homozygote
Hot Temperature
Mosaicism
Protein Kinases / genetics
Regulatory Sequences, Nucleic Acid
Transcription Factors / genetics*,  physiology
Vulva / cytology,  embryology*
Chemical
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/DNA-Binding Proteins; 0/Helminth Proteins; 0/LIN-25 protein, C elegans; 0/Sur-2 protein, C elegans; 0/Transcription Factors; EC 2.7.-/Protein Kinases

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