Document Detail


The Caenorhabditis elegans gene lin-26 can trigger epithelial differentiation without conferring tissue specificity.
MedLine Citation:
PMID:  11437447     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
How epithelial cell fates become specified is poorly understood. We have previously shown that the putative C2H2 zinc-finger transcription factor LIN-26 is required for the differentiation of ectodermal and mesodermal epithelial cells in Caenorhabditis elegans. Here, we report that ectopic LIN-26 expression during early gastrulation transforms most blastomeres into epithelial-like cells. Specifically, LIN-26 induced the expression of three epithelial markers: the adherens junction protein JAM-1; DLG-1, which is essential for the assembly of JAM-1 at junctions; and CHE-14, which is involved in apical trafficking. Furthermore, ultrastructural studies revealed that ectopic LIN-26 expression induced the formation of adherens-like junctions. However, ectopic lin-26 expression did not confer any tissue-specific cell fate, such as the epidermal cell fate, as evidenced from the observation that several epidermal-specific genes were not induced. Conversely, we show that epidermal cells displayed some polarity defects in lin-26 mutants. We conclude that lin-26 can induce epithelial differentiation and that epitheliogenesis is not a default pathway in C. elegans.
Authors:
S Quintin; G Michaux; L McMahon; A Gansmuller; M Labouesse
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental biology     Volume:  235     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  2001 Jul 
Date Detail:
Created Date:  2001-07-04     Completed Date:  2001-08-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  410-21     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Affiliation:
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, F-67404 Illkirch Cedex, C.U. de Strasbourg, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified
Blastomeres / metabolism
Caenorhabditis elegans / genetics*
Caenorhabditis elegans Proteins*
Cell Adhesion Molecules*
Cell Differentiation
Cell Lineage
DNA, Complementary / metabolism
DNA-Binding Proteins / genetics*
Drosophila Proteins*
Ectoderm / metabolism
Embryo, Nonmammalian / ultrastructure
Epidermis / embryology,  metabolism
Gastrula / metabolism
Green Fluorescent Proteins
Hot Temperature
Insect Proteins / metabolism
Luminescent Proteins / metabolism
Membrane Proteins / metabolism
Mesoderm / metabolism
Microscopy, Electron
Models, Biological
Phenotype
Receptors, Cell Surface*
Temperature
Transcription Factors / genetics*
Tumor Suppressor Proteins*
Chemical
Reg. No./Substance:
0/AJM-1 protein, C elegans; 0/Caenorhabditis elegans Proteins; 0/Cell Adhesion Molecules; 0/DNA, Complementary; 0/DNA-Binding Proteins; 0/Drosophila Proteins; 0/Insect Proteins; 0/Luminescent Proteins; 0/Membrane Proteins; 0/Receptors, Cell Surface; 0/Transcription Factors; 0/Tumor Suppressor Proteins; 0/lin-26 protein, C elegans; 143513-41-1/discs large 1 protein, Drosophila; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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