Document Detail


The Caenorhabditis elegans ect-2 RhoGEF gene regulates cytokinesis and migration of epidermal P cells.
MedLine Citation:
PMID:  16170304     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A reduction-of-function mutation in ect-2 was isolated as a suppressor of the Multivulva phenotype of a lin-31 mutation. Analysis using markers indicates that this mutation causes defects in both the cytokinesis and migration of epidermal P cells, phenotypes similar to those caused by expressing a rho-1 dominant-negative construct. ect-2 encodes the Caenorhabditis elegans orthologue of the mouse Ect2 and Drosophila Pebble that function as guanine nucleotide exchange factors (GEFs) for Rho GTPases. The ect-2Colon, two colonsGFP reporter is expressed in embryonic cells and P cells. RNA interference of ect-2 causes sterility and embryonic lethality, in addition to the P-cell defects. We have determined the lesions of two ect-2 alleles, and described their differences in phenotypes in specific tissues. We propose a model in which ECT-2GEF not only activates RHO-1 for P-cell cytokinesis, but also collaborates with UNC-73GEF and at least two Rac proteins to regulate P-cell migration.
Authors:
Kiyokazu Morita; Keiko Hirono; Min Han
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  EMBO reports     Volume:  6     ISSN:  1469-221X     ISO Abbreviation:  EMBO Rep.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-01     Completed Date:  2006-02-16     Revised Date:  2013-08-08    
Medline Journal Info:
Nlm Unique ID:  100963049     Medline TA:  EMBO Rep     Country:  England    
Other Details:
Languages:  eng     Pagination:  1163-8     Citation Subset:  IM    
Affiliation:
Department of MCD Biology, Howard Hughes Medical Institute, University of Colorado, Boulder, Colorado 80309, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Caenorhabditis elegans / genetics*,  physiology
Caenorhabditis elegans Proteins / genetics,  physiology
Cell Movement
Chromosome Mapping
Chromosomes
Consensus Sequence
Cytokinesis*
Epithelial Cells / physiology*
Female
Genes, Helminth*
Genes, Reporter
Genetic Markers
Green Fluorescent Proteins / metabolism
Guanine Nucleotide Exchange Factors / genetics,  physiology*
Models, Biological
Molecular Sequence Data
Promoter Regions, Genetic
Protein Structure, Tertiary
Sequence Deletion
Grant Support
ID/Acronym/Agency:
GM47869/GM/NIGMS NIH HHS; R01 GM047869/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/Ect-2 protein, C elegans; 0/Genetic Markers; 0/Guanine Nucleotide Exchange Factors; 0/rho guanine nucleotide exchange factors; 147336-22-9/Green Fluorescent Proteins
Comments/Corrections

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