Document Detail


Caenorhabditis elegans FOS-1 and JUN-1 regulate plc-1 expression in the spermatheca to control ovulation.
MedLine Citation:
PMID:  19570917     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fos and Jun are components of activator protein-1 (AP-1) and play crucial roles in the regulation of many cellular, developmental, and physiological processes. Caenorhabditis elegans fos-1 has been shown to act in uterine and vulval development. Here, we provide evidence that C. elegans fos-1 and jun-1 control ovulation, a tightly regulated rhythmic program in animals. Knockdown of fos-1 or jun-1 blocks dilation of the distal spermathecal valve, a critical step for the entry of mature oocytes into the spermatheca for fertilization. Furthermore, fos-1 and jun-1 regulate the spermathecal-specific expression of plc-1, a gene that encodes a phospholipase C (PLC) isozyme that is rate-limiting for inositol triphosphate production and ovulation, and overexpression of PLC-1 rescues the ovulation defect in fos-1(RNAi) worms. Unlike fos-1, regulation of ovulation by jun-1 requires genetic interactions with eri-1 and lin-15B, which are involved in the RNA interference pathway and chromatin remodeling, respectively. At least two isoforms of jun-1 are coexpressed with fos-1b in the spermatheca, and different AP-1 dimers formed between these isoforms have distinct effects on the activation of a reporter gene. These findings uncover a novel role for FOS-1 and JUN-1 in the reproductive system and establish C. elegans as a model for studying AP-1 dimerization.
Authors:
Susan M Hiatt; Holli M Duren; Y John Shyu; Ronald E Ellis; Naoki Hisamoto; Kunihiro Matsumoto; Ken-Ichi Kariya; Tom K Kerppola; Chang-Deng Hu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2009-07-01
Journal Detail:
Title:  Molecular biology of the cell     Volume:  20     ISSN:  1939-4586     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-09-01     Completed Date:  2009-12-29     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3888-95     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Caenorhabditis elegans* / anatomy & histology,  physiology
Caenorhabditis elegans Proteins / genetics,  metabolism*
Chromatin Assembly and Disassembly
Gene Expression Regulation*
Inositol 1,4,5-Trisphosphate / metabolism
Mutation
Ovulation / physiology*
Phosphoinositide Phospholipase C / genetics,  metabolism*
Protein Isoforms / genetics,  metabolism*
Proto-Oncogene Proteins c-fos / genetics,  metabolism*
Proto-Oncogene Proteins c-jun / genetics,  metabolism*
RNA Interference
Recombinant Fusion Proteins / genetics,  metabolism
Signal Transduction / physiology
Transcription Factor AP-1 / chemistry,  genetics,  metabolism
Grant Support
ID/Acronym/Agency:
R01 GM086213/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/FOS-1 protein, C elegans; 0/Protein Isoforms; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 0/Recombinant Fusion Proteins; 0/Transcription Factor AP-1; 85166-31-0/Inositol 1,4,5-Trisphosphate; EC 3.1.4.11/Phosphoinositide Phospholipase C; EC 3.1.4.11/plc-1 protein, C elegans
Comments/Corrections

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