| Caenorhabditis elegans {Delta}12-desaturase FAT-2 is a bifunctional desaturase able to desaturate a diverse range of fatty acid substrates at the {Delta}12 and {Delta}15 positions. | |
| | |
MedLine Citation:
|
PMID: 22041902 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Caenorhabditis elegans FAT-2 has been characterized as fatty acid Δ12-desaturase able to desaturate C16 and C18 fatty acids. However, in this report we show that when expressed in yeast cells this enzyme can also catalyse Δ15-desaturation. This results in the production of both linoleic acid (ω6 C18:2Δ9,12) and linolenic acid (ω3 C18:3Δ9,12,15) from oleic acid (C18:1Δ9) substrate, hexadecadienolic acid (ω4 C16:2Δ9,12) and hexadecatrienoic acid (ω1 C16:3Δ9,12,15) from palmitoleic acid (C16:1Δ9) substrate. In addition, this enzyme can also produce C14:2Δ9,12, C15:2Δ9,12, C17:2Δ9,12, C18:4Δ6,9,12,15 when C14:1Δ9, C15:1Δ9, C17:1Δ9, C18:3Δ6,9,12 substrates are available in yeast cells. GC-MS analysis of DOMX modification of fatty acid methyl esters confirms the positions of all newly formed double bonds. These results indicate that when expressed in yeast the C. elegans Δ12-desaturase CeFAT-2 shows a characteristic of a bifunctional Δ12/Δ15 desaturase and has a great deal of elasticity with respect to fatty acid chain length in being able to accept fatty acids ranging from C14 to C18. Interestingly, in spite of possessing a bifunctional Δ12/Δ15 desaturation activity, phylogenetic analysis suggests that C. elegans Δ12-desaturase CeFAT-2 might have arisen independently from other reported dual Δ12/Δ15 desaturases from fungi and protozoa. |
| | |
Authors:
|
Xue-Rong Zhou; Allan G Green; Surinder P Singh |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-10-31 |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: - ISSN: 1083-351X ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
|
Created Date: 2011-11-1 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
CSIRO Plant Industry, Australia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Type II Arginine Methyltransferase PRMT5 Regulates the Gene Expression of Inhibitors of Differentiat...
Next Document: Survival Impact of Adjuvant Paclitaxel and Carboplatin for Early-Stage Ovarian Clear-Cell Carcinoma ...