Document Detail


The Caenorhabditis elegans ABL-1 tyrosine kinase is required for Shigella flexneri pathogenesis.
MedLine Citation:
PMID:  16820504     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Shigellosis is a diarrheal disease caused by the gram-negative bacterium Shigella flexneri. Following ingestion of the bacterium, S. flexneri interferes with innate immunity, establishes an infection within the human colon, and initiates an inflammatory response that results in destruction of the tissue lining the gut. Examination of host cell factors required for S. flexneri pathogenesis in vivo has proven difficult due to limited host susceptibility. Here we report the development of a pathogenesis system that involves the use of Caenorhabditis elegans as a model organism to study S. flexneri virulence determinants and host molecules required for pathogenesis. We show that S. flexneri-mediated killing of C. elegans correlates with bacterial accumulation in the intestinal tract of the animal. The S. flexneri virulence plasmid, which encodes a type III secretory system as well as various virulence determinants crucial for pathogenesis in mammalian systems, was found to be required for maximal C. elegans killing. Additionally, we demonstrate that ABL-1, the C. elegans homolog of the mammalian c-Abl nonreceptor tyrosine kinase ABL1, is required for S. flexneri pathogenesis in nematodes. These data demonstrate the feasibility of using C. elegans to study S. flexneri pathogenesis in vivo and provide insight into host factors that contribute to S. flexneri pathogenesis.
Authors:
Elizabeth A Burton; Ann Marie Pendergast; Alejandro Aballay
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  72     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-07-05     Completed Date:  2006-09-05     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5043-51     Citation Subset:  IM    
Affiliation:
Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Caenorhabditis elegans / enzymology*,  microbiology*
Intestines / microbiology
Mutation
Plasmids / genetics
Proto-Oncogene Proteins c-abl / genetics,  metabolism*
Shigella flexneri / genetics,  pathogenicity*
Virulence / genetics
Grant Support
ID/Acronym/Agency:
AI065641/AI/NIAID NIH HHS; CA009111-27/CA/NCI NIH HHS; CA70940/CA/NCI NIH HHS; GM62375/GM/NIGMS NIH HHS; GM70977/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
EC 2.7.10.2/Proto-Oncogene Proteins c-abl
Comments/Corrections

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