Document Detail

Cadmium and zinc flux in wild-type and cadmium-resistant CHO cells.
MedLine Citation:
PMID:  24263366     Owner:  NLM     Status:  Publisher    
Cellular flux of cadmium-109 and zinc-65 is characterized in cultured Chinese hamster ovary cells. The transport of cadmium is primarily unidirectional and, following uptake, cadmium is strongly retained. Zinc transport is bidirectional and intracellular zinc continuously leaches out into the medium. Nonradioactive cadmium or zinc enhances the efflux of(65)Zn from prelabeled cells. Transport of these metals into wild-type cells is not affected by azide, ouabain, cycloheximide, or actinomycin D. A cadmium-resistant mutant was isolated that exhibited altered sensitivities to certain inhibitors of macromolecular synthesis as well as quantitative differences in metal transport and accumulation. Although the mutant accumulates less cadmium than the wild-type cell, that which is retained is bound much more tightly. In addition, this lower rate of cadmium uptake is significantly decreased by either cycloheximide or actinomycin D. This suggests that thede novo synthesis of a protein or proteins is required for much of the net cadmium retention by the cadmium-resistant cells.
Alice J Corrigan; P C Huang
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Publication Detail:
Journal Detail:
Title:  Biological trace element research     Volume:  5     ISSN:  1559-0720     ISO Abbreviation:  Biol Trace Elem Res     Publication Date:  1983 Feb 
Date Detail:
Created Date:  2013-11-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7911509     Medline TA:  Biol Trace Elem Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  25-33     Citation Subset:  -    
Department of Biochemistry, The Johns Hopkins University, School of Hygiene and Public Health, 615 North Wolfe Street, 21205, Baltimore, Maryland.
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