| Cadmium toxicity in the isolated perfused rat liver. | |
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MedLine Citation:
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PMID: 3787665 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Isolated perfused livers from male and female Sprague-Dawley rats were exposed to cadmium chloride (50 and 200 microM). Acute hepatotoxicity was investigated by measuring cadmium-induced changes in bile flow, urea synthesis and alanine aminotransferase (ALT) leakage. Cadmium-induced lipid peroxidation was estimated by formation of conjugated dieners and thiobarbituric acid (TBA) reactants. Cadmium, at both concentrations, caused a rapid decrease in bile flow (within 40 min) and complete cholestasis within 70 min exposure in livers perfused from both male and female rats. Cadmium exposure (50 and 200 microM) also resulted in the leakage of ALT into the perfusate within 60 min. In contrast, exposure of isolated rat hepatocytes to as high as 500 microM cadmium did not result in enzyme leakage until 180 min exposure. Sex differences in cadmium-induced cholestasis and ALT leakage were not observed at these concentrations. Malondialdehyde was not detected in the perfusate nor were conjugated dienes detected in liver tissue following 90 min cadmium exposure. These data demonstrate that the isolated perfused rat liver (IPRL) is a sensitive system in which to study chemically induced hepatotoxicity. Cadmium rapidly causes functional alterations and cellular damage in perfused livers from both male and female rats. Cadmium-induced liver injury was apparently not related to lipid peroxidation. |
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Authors:
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S Lupo; W R Hewitt; G F Rush |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Toxicology letters Volume: 34 ISSN: 0378-4274 ISO Abbreviation: Toxicol. Lett. Publication Date: 1986 Nov |
Date Detail:
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Created Date: 1987-01-12 Completed Date: 1987-01-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7709027 Medline TA: Toxicol Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 5-11 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alanine Transaminase
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metabolism Animals Bile / drug effects, secretion Cadmium / toxicity* Female L-Lactate Dehydrogenase / metabolism Lipid Peroxides / biosynthesis Liver / drug effects*, metabolism Male Perfusion Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
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0/Lipid Peroxides; 7440-43-9/Cadmium; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 2.6.1.2/Alanine Transaminase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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