Document Detail

Cadmium ion-induced alterations of phospholipid metabolism in endothelial cells.
MedLine Citation:
PMID:  1910817     Owner:  NLM     Status:  MEDLINE    
Cadmium exposure is capable of causing acute and chronic lung injuries, but the specific pathogenetic mechanisms are uncertain. The effects of cadmium ion (Cd2+) on phospholipid metabolism were examined in cultured bovine pulmonary artery endothelial cells (BPAEC), as endothelial cells appear to be particularly vulnerable to the toxic effects of this metallic ion. Exposure of radiolabeled BPAEC to millimolar concentrations of Cd2+ causes liberation of substantial amounts of [3H]arachidonic acid ([3H]AA), but only small amounts of [14C]stearic acid, from each of the major phospholipid subclasses. Analyses of hydrolytic products in BPAEC radiolabeled with [3H]myo-inositol and exposed to Cd2+ indicate that degradation of complex phospholipids is mediated by phospholipase A2. The ability of BPAEC to incorporate fatty acids or lysophosphatides into complex phospholipids is similarly impaired after exposure to Cd2+, suggesting that the liberation of [3H]AA might be due to impairment of reacylation mechanisms and not to increased hydrolytic activity of phospholipase A2. Of the two enzymes involved in reacylation reactions, Cd2+ is found to inhibit the activity of arachidonyl-specific acyl coenzyme A synthetase but not the activity of acyltransferase. Cd2+ also causes a profound time- and dose-dependent depletion of adenosine triphosphate levels in BPAEC, and these changes closely correlate with the liberation of [3H]AA. We suggest that impairment of reacylation mechanisms, and the consequent accumulation of arachidonic acid, may be important in the development of the acute inflammatory reaction that is characteristic of Cd(2+)-induced lung injury.
J M Nelson; P G Duane; K L Rice; D E Niewoehner
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of respiratory cell and molecular biology     Volume:  5     ISSN:  1044-1549     ISO Abbreviation:  Am. J. Respir. Cell Mol. Biol.     Publication Date:  1991 Oct 
Date Detail:
Created Date:  1991-11-15     Completed Date:  1991-11-15     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8917225     Medline TA:  Am J Respir Cell Mol Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  328-36     Citation Subset:  IM    
Pulmonary Section-111N, Veterans Administration Medical Center, Minneapolis, MN 55417.
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MeSH Terms
Adenosine Triphosphate / metabolism
Arachidonic Acid / metabolism
Cadmium / toxicity*
Cations, Divalent
Cell Survival / drug effects
Cells, Cultured
Chromatography, High Pressure Liquid
Endothelium, Vascular / cytology,  drug effects,  metabolism*
Fatty Acids, Nonesterified / metabolism
Inositol / metabolism
Lysophosphatidylcholines / metabolism
Phospholipases A / metabolism
Phospholipases A2
Phospholipids / metabolism*
Stearic Acids / metabolism
Reg. No./Substance:
0/Cations, Divalent; 0/Fatty Acids, Nonesterified; 0/Lysophosphatidylcholines; 0/Phospholipids; 0/Stearic Acids; 506-32-1/Arachidonic Acid; 56-65-5/Adenosine Triphosphate; 57-11-4/stearic acid; 6917-35-7/Inositol; 7440-43-9/Cadmium; EC 3.1.1.-/Phospholipases A; EC A2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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