Document Detail

Cadmium induces transcription of proto-oncogenes c-jun and c-myc in rat L6 myoblasts.
MedLine Citation:
PMID:  1696944     Owner:  NLM     Status:  MEDLINE    
The effect of cadmium on the expression of oncogenes c-jun, c-fos, and c-myc was studied by exposing L6J1 myoblast cultures to different doses of cadmium chloride and then analyzing the abundance of oncogene transcripts. Cadmium induced a transient accumulation of c-jun and c-myc mRNA with maximum expression at 2-4 h. At the same time, the level of c-fos transcripts remained below the detection level. Both the c-fos and c-jun genes could. However, be induced by treating the myoblasts with insulin. Cadmium induction of c-jun and c-myc mRNA occurred in a concentration-dependent manner with maximum stimulation at 5-10 microM. In the presence of cycloheximide, c-jun and c-myc genes were superinduced by the addition of cadmium. Under these conditions there was also a marked increase in c-fos transcripts. Induction of c-myc and c-jun by cadmium and c-fos by a combination of cadmium and cycloheximide could be abolished by blocking transcription with actinomycin D. The cadmium-induced increase in c-jun and c-myc mRNA was enhanced in myoblasts stably transfected with a mouse c-fos gene under a metallothionein promoter. Our present data suggest that cadmium has the potential to deregulate the expression of several important oncogenes.
P Jin; N R Ringertz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  265     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1990 Aug 
Date Detail:
Created Date:  1990-09-27     Completed Date:  1990-09-27     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  14061-4     Citation Subset:  IM    
Department of Medical Cell Genetics, Medical Nobel Institute, Karolinska Institutet, Stockholm, Sweden.
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MeSH Terms
Cadmium / pharmacology*
Cadmium Chloride
Cell Division / drug effects
Cell Line
DNA-Binding Proteins / genetics*
Protein-Tyrosine Kinases / genetics
Proto-Oncogene Proteins / genetics*
Proto-Oncogene Proteins c-jun
Proto-Oncogene Proteins c-myc
RNA / genetics,  isolation & purification
RNA, Messenger / genetics*,  isolation & purification
Restriction Mapping
Transcription Factors / genetics*
Transcription, Genetic / drug effects*
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-jun; 0/Proto-Oncogene Proteins c-myc; 0/RNA, Messenger; 0/Transcription Factors; 10108-64-2/Cadmium Chloride; 63231-63-0/RNA; 7440-43-9/Cadmium; EC Kinases

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