Document Detail


Cadmium-induced inflammatory responses in cells relevant for lung toxicity: Expression and release of cytokines in fibroblasts, epithelial cells and macrophages.
MedLine Citation:
PMID:  20105457     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Inhalation is an important route of cadmium (Cd) exposure, and the lung is considered to be one of the main target organs of Cd toxicity. Pulmonary inflammation seems to be involved in development of many lung diseases. In the present study we show that Cd(2+) at fairly low concentrations affects gene expression of several different cytokines/chemokines in human M1 fibroblasts. The chemokines CXCL2, CXCL3, IL-8/CXCL8 and CCL26, the pro-inflammatory cytokine IL-6 and the receptor IL-1RL1 were expressed at high levels after exposure to 7 microM Cd(2+) for 7h. The expression of some important cytokines was further studied in two different primary cell cultures from rat lungs. Cd(2+) induced cytokine responses at low concentrations (3-6 microM) and early time-points both in type 2 epithelial cell-enriched cultures and alveolar macrophages. However, the two primary lung cells displayed different patterns of cytokine release. Cd(2+) induced an increased release of IL-6 and MIP-2/CXCL2 from the epithelial cells and MIP-2, IL-1beta and TNF-alpha from alveolar macrophages. In conclusion, the marked up-regulation of different cytokines in these cell types, that are important in development of lung injury and disease, suggests that inflammation may contribute in Cd-induced lung damage.
Authors:
Marit L?g; Dmitrii Rodionov; Johan Ovrevik; Oddmund Bakke; Per E Schwarze; Magne Refsnes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-25
Journal Detail:
Title:  Toxicology letters     Volume:  193     ISSN:  1879-3169     ISO Abbreviation:  Toxicol. Lett.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-11     Completed Date:  2010-03-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7709027     Medline TA:  Toxicol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  252-60     Citation Subset:  IM    
Copyright Information:
2010 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Norwegian Institute of Public Health, Oslo, Norway. marit.lag@fhi.no
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MeSH Terms
Descriptor/Qualifier:
Animals
Cadmium / toxicity*
Cell Survival / drug effects
Cytokines / genetics*,  secretion
Epithelial Cells / drug effects,  metabolism
Fibroblasts / drug effects,  metabolism
Inflammation / chemically induced*
Interleukin-1beta / genetics
Lung / drug effects*
Macrophages / drug effects,  metabolism
Male
Oligonucleotide Array Sequence Analysis
Polymerase Chain Reaction
Rats
Rats, Inbred WKY
Tumor Necrosis Factor-alpha / genetics
Chemical
Reg. No./Substance:
0/Cytokines; 0/Interleukin-1beta; 0/Tumor Necrosis Factor-alpha; 7440-43-9/Cadmium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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