| Cadmium affects tobacco cells by a series of three waves of reactive oxygen species that contribute to cytotoxicity. | |
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MedLine Citation:
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PMID: 16930321 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cadmium is suspected to exert its toxic action on cells through oxidative damage. However, the transition metal is unable to directly generate reactive oxygen species (ROS) via redox reactions with molecular oxygen in a biological environment. Here, we show that bright yellow-2 (BY-2) tobacco cells exposed to millimolar concentrations of CdCl(2) developed cell death within 2-3 h. The death process was preceded by two successive waves of ROS differing in their nature and subcellular localization. Firstly, these consisted in the transient NADPH oxidase-dependent accumulation of H(2)O(2) followed by the accumulation of O(2) (-*) in mitochondria. A third wave of ROS consisting in fatty acid hydroperoxide accumulation was concomitant with cell death. Accumulation of H(2)O(2) was preceded by an increase in cytosolic free calcium concentration originating from internal pools that was essential to activate the NADPH oxidase. The cell line gp3, impaired in NADPH oxidase activity, and that was unable to accumulate H(2)O(2) in response to Cd(2+), was nevertheless poisoned by the metal. Therefore, this first wave of ROS was not sufficient to trigger all the cadmium-dependent deleterious effects. However, we show that the accumulation of O(2) (-*) of mitochondrial origin and membrane peroxidation are key players in Cd(2+)-induced cell death. |
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Authors:
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Lionel Garnier; Françoise Simon-Plas; Patrice Thuleau; Jean-Pierre Agnel; Jean-Pierre Blein; Raoul Ranjeva; Jean-Luc Montillet |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Plant, cell & environment Volume: 29 ISSN: 0140-7791 ISO Abbreviation: Plant Cell Environ. Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-08-25 Completed Date: 2006-11-21 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9309004 Medline TA: Plant Cell Environ Country: United States |
Other Details:
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Languages: eng Pagination: 1956-69 Citation Subset: IM |
Affiliation:
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Commissariat à l'Energie Atomique, Centre de Cadarache, DSV-DEVM, Laboratoire de Radiobiologie Végétale, 13108 Saint-Paul lez Durance Cedex, France. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/S71335 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Cadmium / pharmacology* Calcium / metabolism Calcium-Calmodulin-Dependent Protein Kinases / metabolism Cytoplasm / metabolism DNA Primers Homeostasis Hydrogen Peroxide / metabolism Molecular Sequence Data NADPH Oxidase / metabolism RNA Processing, Post-Transcriptional Reactive Oxygen Species / metabolism* Tobacco / cytology, drug effects*, growth & development, metabolism |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/Reactive Oxygen Species; 7440-43-9/Cadmium; 7440-70-2/Calcium; 7722-84-1/Hydrogen Peroxide; EC 1.6.3.1/NADPH Oxidase; EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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