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CDH3/P-Cadherin regulates migration of HuCCT1 cholangiocarcinoma cells.
MedLine Citation:
PMID:  21189991     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Intrahepatic cholangiocarcinoma is the second most common subtype of primary hepatobilliary cancer. Despite advances in surgical and medical therapy, its survival rate remains poor. Compared to hepatocellular carcinoma (HCC), the most common liver malignancy, the underlying mechanisms of cholangiocarcinoma carcinogenesis are poorly characterized. P-cadherin (CDH3) is a cadherin super family member. Although CDH3 is frequently over-expressed in cholangiocarcinoma tissues, its roles have never been characterized. To determine the roles of CDH3 in cholangiocarcinoma, we investigated CDH3 function in HuCCT1 cells using specific siRNA. Transfection with CDH3 siRNA did not affect proliferation of HuCCT1 cells. However, cell migration and invasion were significantly reduced when CDH3 was down-regulated. In addition, expressions of several biomarkers for epithelial-mesenchymal transition (EMT) were not changed by CDH3 down-regulation. These results suggest that CDH3 regulates cell migration independent of EMT in cholangiocarcinoma cells.
Authors:
Sungmin Baek; Yong-Whan Lee; Sik Yoon; Sun-Yong Baek; Bong-Seon Kim; Sae-Ock Oh
Publication Detail:
Type:  Journal Article     Date:  2010-06-30
Journal Detail:
Title:  Anatomy & cell biology     Volume:  43     ISSN:  2093-3673     ISO Abbreviation:  Anat Cell Biol     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-12-30     Completed Date:  2011-07-14     Revised Date:  2011-07-19    
Medline Journal Info:
Nlm Unique ID:  101531987     Medline TA:  Anat Cell Biol     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  110-7     Citation Subset:  -    
Affiliation:
Department of Anatomy, School of Medicine, Pusan National University, Yangsan, Korea.
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