| Cadherin-catenin complexes during zebrafish oogenesis: heterotypic junctions between oocytes and follicle cells. | |
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MedLine Citation:
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PMID: 10456847 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During vertebrate oogenesis, the germ cells and associated somatic cells remain connected by a variety of adhering junctional complexes. However, the molecular composition of these cellular structures is largely unknown. To identify the proteins forming the heterotypic adherens junctions between oocytes and follicle cells in the zebrafish (Danio rerio), the cDNAs encoding alphaE-catenin and plakoglobin were isolated. Using these cDNAs, in combination with the previously isolated beta-catenin cDNA, and antibodies specific for alpha- and beta-catenin, plakoglobin, and N- and E-cadherin, we found differences in catenin and plakoglobin gene expression during oogenesis. The immunolocalization of these plaque proteins, as well as of cadherins, in the ovarian follicle indicated an enrichment of alpha- and beta-catenin and of E-cadherin-like protein(s) in the oocyte cortex, notably at sites of oocyte-follicle cell contacts, suggesting the presence of hitherto unknown heterotypic adherens junctions between these cells. By contrast, plakoglobin and N-cadherin localization was restricted to cell-cell contacts in the follicle cell layer. During oocyte maturation, mRNAs for alphaE- and beta-catenin and plakoglobin accumulated, and all three plaque-forming proteins were stored in unfertilized eggs, either in complexed forms with cadherins or as free cytoplasmic pools. These findings suggest possible roles of these junctional proteins during early embryogenesis. |
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Authors:
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J Cerdà; S Reidenbach; S Prätzel; W W Franke |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biology of reproduction Volume: 61 ISSN: 0006-3363 ISO Abbreviation: Biol. Reprod. Publication Date: 1999 Sep |
Date Detail:
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Created Date: 1999-09-20 Completed Date: 1999-09-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 692-704 Citation Subset: IM |
Affiliation:
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Division of Cell Biology, German Cancer Research Center, D-69120 Heidelberg, Germany. waken@ctv.es |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AF099737; AF099738 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Antibody Specificity Cadherins / metabolism* Cytoskeletal Proteins / chemistry, genetics, metabolism* DNA, Complementary / isolation & purification Desmoplakins Female Humans Immunoblotting Intercellular Junctions* Mice Microscopy, Electron Molecular Sequence Data Oocytes / chemistry, ultrastructure* Oogenesis* Ovarian Follicle / chemistry, ultrastructure* Sequence Homology Trans-Activators* Zebrafish / physiology* Zebrafish Proteins alpha Catenin beta Catenin gamma Catenin |
| Chemical | |
Reg. No./Substance:
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0/CTNNA1 protein, human; 0/CTNNB1 protein, human; 0/Cadherins; 0/Catna1 protein, mouse; 0/Catnb protein, mouse; 0/Cytoskeletal Proteins; 0/DNA, Complementary; 0/Desmoplakins; 0/Trans-Activators; 0/Zebrafish Proteins; 0/alpha Catenin; 0/beta Catenin; 0/ctnnb protein, zebrafish; 0/gamma Catenin |
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