Document Detail


CadF expression in Campylobacter jejuni strains incubated under low-temperature water microcosm conditions which induce the viable but non-culturable (VBNC) state.
MedLine Citation:
PMID:  23314927     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Campylobacter jejuni is a major gastrointestinal pathogen that colonizes host mucosa via interactions with extracellular matrix proteins such as fibronectin. The aim of this work was to study in vitro the adhesive properties of C. jejuni ATCC 33291 and C. jejuni 241 strains, in both culturable and viable but non-culturable (VBNC) forms. To this end, the expression of the outer-membrane protein CadF, which mediates C. jejuni binding to fibronectin, was evaluated. VBNC bacteria were obtained after 46-48 days of incubation in freshwater at 4 °C. In both cellular forms, the expression of the cadF gene, assessed at different time points by RT-PCR, was at high levels until the third week of VBNC induction, while the intensity of the signal declined during the last stage of incubation. CadF protein expression by the two C. jejuni strains was analysed using 2-dimensional electrophoresis and mass spectrometry; the results indicated that the protein, although at low levels, is also present in the VBNC state. Adhesion assays with culturable and VBNC cells, evaluated on Caco-2 monolayers, showed that non-culturable bacteria retain their ability to adhere to intestinal cells, though at a reduced rate. Our results demonstrate that the C. jejuni VBNC population maintains an ability to adhere and this may thus have an important role in the pathogenicity of this microorganism.
Authors:
Vania Patrone; Raffaella Campana; Luciana Vallorani; Sabrina Dominici; Sara Federici; Lucia Casadei; Anna Maria Gioacchini; Vilberto Stocchi; Wally Baffone
Related Documents :
23036607 - Rat neural stem cell proliferation and differentiation are durably altered by the in ut...
24221877 - Enrichment for nicotiana heterokaryons after protoplast fusion and subsequent growth in...
23725837 - Effects of salvianolic acid and notoginseng triterpenes on angiogenesis in ea-hy926 cel...
6199837 - Permissive effect of substratum contact on dna synthesis in concanavalin a-treated lymp...
22034857 - Translating human embryonic stem cells from 2d to 3d cultures in a defined medium on la...
1872907 - Active cholesterol biosynthesis in cultured aortic smooth muscle cells: evolution durin...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-13
Journal Detail:
Title:  Antonie van Leeuwenhoek     Volume:  -     ISSN:  1572-9699     ISO Abbreviation:  Antonie Van Leeuwenhoek     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372625     Medline TA:  Antonie Van Leeuwenhoek     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Division of Toxicology, Hygienic and Environmental Sciences Department of Biomolecular Sciences, University of Urbino "Carlo Bo", Via S. Chiara 27, 61029, Urbino, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Therapeutic gene targeting approaches for the treatment of dyslipidemias and atherosclerosis.
Next Document:  Halomonas nanhaiensis sp. nov., a halophilic bacterium isolated from a sediment sample from the Sout...