Document Detail


A Cacna1a knockin migraine mouse model with increased susceptibility to cortical spreading depression.
MedLine Citation:
PMID:  15003170     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Migraine is a common, disabling, multifactorial, episodic neurovascular disorder of unknown etiology. Familial hemiplegic migraine type 1 (FHM-1) is a Mendelian subtype of migraine with aura that is caused by missense mutations in the CACNA1A gene that encodes the alpha(1) subunit of neuronal Ca(v)2.1 Ca(2+) channels. We generated a knockin mouse model carrying the human pure FHM-1 R192Q mutation and found multiple gain-of-function effects. These include increased Ca(v)2.1 current density in cerebellar neurons, enhanced neurotransmission at the neuromuscular junction, and, in the intact animal, a reduced threshold and increased velocity of cortical spreading depression (CSD; the likely mechanism for the migraine aura). Our data show that the increased susceptibility for CSD and aura in migraine may be due to cortical hyperexcitability. The R192Q FHM-1 mouse is a promising animal model to study migraine mechanisms and treatments.
Authors:
Arn M J M van den Maagdenberg; Daniela Pietrobon; Tommaso Pizzorusso; Simon Kaja; Ludo A M Broos; Tiziana Cesetti; Rob C G van de Ven; Angelita Tottene; Jos van der Kaa; Jaap J Plomp; Rune R Frants; Michel D Ferrari
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuron     Volume:  41     ISSN:  0896-6273     ISO Abbreviation:  Neuron     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-03-08     Completed Date:  2004-04-12     Revised Date:  2012-02-22    
Medline Journal Info:
Nlm Unique ID:  8809320     Medline TA:  Neuron     Country:  United States    
Other Details:
Languages:  eng     Pagination:  701-10     Citation Subset:  IM    
Affiliation:
Department of Biomedical Sciences, University of Padova, CNR Institute of Neuroscience, Viale G. Colombo 3, 35121 Padova, Italy. dani@civ.bio.unipd.it
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium Channels / biosynthesis,  genetics*
Calcium Channels, P-Type
Calcium Channels, Q-Type
Cells, Cultured
Cortical Spreading Depression / genetics*
Disease Models, Animal*
Female
Genetic Predisposition to Disease*
Humans
Male
Mice
Mice, Mutant Strains
Mice, Transgenic
Migraine with Aura / genetics*,  metabolism
Motor Endplate / genetics,  metabolism
Mutation
Recombination, Genetic*
Grant Support
ID/Acronym/Agency:
E.0847//Telethon; E.1297//Telethon
Chemical
Reg. No./Substance:
0/CACNA1A protein, human; 0/Cacna1a protein, mouse; 0/Calcium Channels; 0/Calcium Channels, P-Type; 0/Calcium Channels, Q-Type
Comments/Corrections
Comment In:
Neuron. 2004 Mar 4;41(5):679-80   [PMID:  15003166 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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