Document Detail


Cabbage lipid transfer protein Bra o 3 is a major allergen responsible for cross-reactivity between plant foods and pollens.
MedLine Citation:
PMID:  16751008     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Food IgE-mediated allergy to members of the Brassicaceae family has been increasingly reported. OBJECTIVE: To characterize cabbage-Brassica oleracea var capitata-allergy and its major allergens. METHODS: A prospective study was performed, recruiting 17 patients allergic to cabbage, and control subjects. Skin prick tests and double-blind placebo-controlled food challenges were performed. A major allergen was isolated from cabbage by RP-HPLC and characterized by N-terminal amino acid sequencing and matrix-assisted laser desorption/ionization mass spectrometry analysis. Specific IgE determinations, IgE immunoblots, and CAP-inhibition assays were also performed. RESULTS: Skin prick test and specific IgE were positive to cabbage in all patients. Five of them referred anaphylactic reactions when eating cabbage, and in another 5 patients, cabbage allergy was further confirmed by double-blind placebo-controlled food challenge. Most of them showed associated sensitizations to mugwort pollen, mustard, and peach. A 9-kd cabbage IgE-binding protein, Bra o 3, was identified as a lipid transfer protein (LTP) with 50% of identity to peach LTP Pru p 3. Skin prick test with Bra o 3 showed positive results in 12 of 14 cases (86%). On CAP inhibition assays, Bra o 3 managed to inhibit significantly the IgE binding to cabbage, mugwort pollen, and peach. Both Bra o 3 and Pru p 3 were recognized by IgE from the patients' sera. CONCLUSION: Bra o 3, a cabbage LTP, is a major allergen in this food, cross-reacting with mugwort pollen and with other plant foods, such as peach. CLINICAL IMPLICATIONS: Cabbage IgE-mediated allergy is a potentially severe condition that can present with other plant food and pollen allergies.
Authors:
Aránzazu Palacín; Jose Cumplido; Javier Figueroa; Oussama Ahrazem; Rosa Sánchez-Monge; Teresa Carrillo; Gabriel Salcedo; Carlos Blanco
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Publication Detail:
Type:  Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't     Date:  2006-03-31
Journal Detail:
Title:  The Journal of allergy and clinical immunology     Volume:  117     ISSN:  0091-6749     ISO Abbreviation:  J. Allergy Clin. Immunol.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-06-05     Completed Date:  2006-07-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1275002     Medline TA:  J Allergy Clin Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1423-9     Citation Subset:  AIM; IM    
Affiliation:
Departamento de Biotecnología, Escuela Técnica Superior Ingenieros Agrónomos, Universidad Politécnica de Madrid, Madrid, Spain.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Allergens / immunology*,  metabolism
Antigens, Plant
Brassica / immunology*
Carrier Proteins / immunology*,  metabolism
Cross Reactions
Double-Blind Method
Female
Food Hypersensitivity / immunology*,  metabolism
Humans
Immunoglobulin E / metabolism
Male
Plant Proteins / immunology*,  metabolism
Pollen / immunology*,  metabolism
Prospective Studies
Protein Binding / immunology
Skin Tests
Chemical
Reg. No./Substance:
0/Allergens; 0/Antigens, Plant; 0/Bra o 3 allergen, Brassica oleracea; 0/Carrier Proteins; 0/Plant Proteins; 0/lipid transfer proteins, plant; 37341-29-0/Immunoglobulin E

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