Document Detail


Ca2+ waves and intracellular Ca2+ concentration in guinea pig and rat myocytes.
MedLine Citation:
PMID:  7830331     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We measured [Ca2+]i of guinea pig and rat myocytes with Ca2+ waves, using fura-2 fluorescence image processing. In guinea pig myocytes, Ca2+ waves were absent during the control perfusion period, but could be induced by the addition of strophanthidin (100 microM) or sodium cyanide (NaCN: 2 mM) to the perfusate. The [Ca2+]i increased from the control values of 69 +/- 5 nM and 46 +/- 2 nM, to 263 +/- 9 (p < 0.05 vs. control) nM and 225 +/- 20 (p < 0.05) nM, respectively, when cells exhibited Ca2+ waves. Although 13% (16 of 121) of the rat myocytes displayed Ca2+ waves during the control perfusion, the [Ca2+]i with Ca2+ waves (56 +/- 9 nM) did not differ from [Ca2+]i in the absence of Ca2+ waves (54 +/- 3 nM). Ca2+ waves were induced by the perfusion with a high Ca2+ solution (24.5 microM) or NaCN (2 microM), and [Ca2+]i increased from the control values of 67 +/- 11 nM and 74 +/- 5 nM, to 231 +/- 41 (p < 0.05 vs. control) nM and 266 +/- 64 nM, respectively, when cells exhibited Ca2+ waves. The Ca2+ waves were abolished by the removal of extracellular Ca2+, or by the perfusion with ryanodine (10 microM) or caffeine (20 mM). In conclusion, it was shown that Ca2+ waves were due to oscillatory Ca2+ release and that the absolute value of [Ca2+]i is important for the appearance of Ca2+ waves in guinea pig and rat myocytes.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
H Hayashi; H Miyata; H Terada; H Satoh; H Katoh; T Nakamura; A Kobayashi
Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Japanese heart journal     Volume:  35     ISSN:  0021-4868     ISO Abbreviation:  Jpn Heart J     Publication Date:  1994 Sep 
Date Detail:
Created Date:  1995-02-22     Completed Date:  1995-02-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0401175     Medline TA:  Jpn Heart J     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  673-82     Citation Subset:  IM    
Affiliation:
Photon Medical Research Center, Hamamatsu University School of Medicine, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Caffeine / pharmacology
Calcium / metabolism*,  physiology
Guinea Pigs
Heart / drug effects
Heart Ventricles / cytology,  drug effects
Image Processing, Computer-Assisted
Male
Microscopy, Fluorescence
Myocardium / cytology,  metabolism*
Rats
Rats, Wistar
Sarcoplasmic Reticulum / metabolism
Sodium Cyanide / pharmacology
Species Specificity
Strophanthidin / pharmacology
Chemical
Reg. No./Substance:
143-33-9/Sodium Cyanide; 58-08-2/Caffeine; 66-28-4/Strophanthidin; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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