| Ca(2+)-synaptotagmin directly regulates t-SNARE function during reconstituted membrane fusion. | |
| | |
MedLine Citation:
|
PMID: 16565726 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In nerve terminals, exocytosis is mediated by SNARE proteins and regulated by Ca(2+) and synaptotagmin-1 (syt). Ca(2+) promotes the interaction of syt with anionic phospholipids and the target membrane SNAREs (t-SNAREs) SNAP-25 and syntaxin. Here, we have used a defined reconstituted fusion assay to determine directly whether syt-t-SNARE interactions couple Ca(2+) to membrane fusion by comparing the effects of Ca(2+)-syt on neuronal (SNAP-25, syntaxin and synaptobrevin) and yeast (Sso1p, Sec9c and Snc2p) SNAREs. Ca(2+)-syt aggregated neuronal and yeast SNARE liposomes to similar extents via interactions with anionic phospholipids. However, Ca(2+)-syt was able to bind and stimulate fusion mediated by only neuronal SNAREs and had no effect on yeast SNAREs. Thus, Ca(2+)-syt regulates fusion through direct interactions with t-SNAREs and not solely through aggregation of vesicles. Ca(2+)-syt drove assembly of SNAP-25 onto membrane-embedded syntaxin, providing direct evidence that Ca(2+)-syt alters t-SNARE structure. |
| | |
Authors:
|
Akhil Bhalla; Michael C Chicka; Ward C Tucker; Edwin R Chapman |
Related Documents
:
|
12684056 - Imidazoline nnc77-0074 stimulates ca2+-evoked exocytosis in ins-1e cells by a phospholi... 21994476 - Increased expression of atp-sensitive k channels improves the right ventricular toleran... 11011146 - Calcium-dependent and -independent hetero-oligomerization in the synaptotagmin family. 16612626 - Effects of dibucaine on the endocytic/exocytic pathways in trypanosoma cruzi. 11152716 - Agatoxin-iva-sensitive calcium channels mediate the presynaptic and postsynaptic nicoti... 16103276 - Investigating the putative glycine hinge in shaker potassium channel. |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2006-03-26 |
Journal Detail:
|
Title: Nature structural & molecular biology Volume: 13 ISSN: 1545-9993 ISO Abbreviation: Nat. Struct. Mol. Biol. Publication Date: 2006 Apr |
Date Detail:
|
Created Date: 2006-05-22 Completed Date: 2006-06-15 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 101186374 Medline TA: Nat Struct Mol Biol Country: United States |
Other Details:
|
Languages: eng Pagination: 323-30 Citation Subset: IM |
Affiliation:
|
Howard Hughes Medical Institute, University of Wisconsin, 1300 University Avenue, SMI 129, Madison, Wisconsin, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Calcium / metabolism* Exocytosis Fungal Proteins / genetics, metabolism Liposomes Membrane Fusion / physiology* Models, Biological Mutagenesis, Site-Directed Nerve Endings / metabolism Rats Recombinant Proteins / genetics, metabolism SNARE Proteins / genetics, metabolism* Synaptosomal-Associated Protein 25 / genetics, metabolism Synaptotagmin I / genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
GM 56827/GM/NIGMS NIH HHS; MH61876/MH/NIMH NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Fungal Proteins; 0/Liposomes; 0/Recombinant Proteins; 0/SNARE Proteins; 0/Synaptosomal-Associated Protein 25; 0/Synaptotagmin I; 0/Syt1 protein, rat; 7440-70-2/Calcium |
| Comments/Corrections | |
Comment In:
|
Nat Struct Mol Biol. 2006 Apr;13(4):301-3
[PMID:
16715046
]
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Structure and mechanism of a bacterial beta-glucosaminidase having O-GlcNAcase activity.
Next Document: Generation of cloned transgenic pigs rich in omega-3 fatty acids.