| A Ca2+-independent receptor for alpha-latrotoxin, CIRL, mediates effects on secretion via multiple mechanisms. | |
| | |
MedLine Citation:
|
PMID: 9526008 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
alpha-Latrotoxin (alpha-Ltx), a component of black widow spider venom, stimulates secretion from nerve terminals and from PC12 cells. In this study we examine the effects of expression of a newly cloned Ca2+-independent receptor for alpha-Ltx (CIRL) on secretion from bovine chromaffin cells. We first characterized the effect of alpha-Ltx on secretion from untransfected cells. alpha-Ltx, by binding in a Ca2+-independent manner to an endogenous receptor, causes subsequent Ca2+-dependent secretion from intact cells. The stimulation of secretion is correlated with Ca2+ influx caused by the toxin. In permeabilized cells in which the Ca2+ concentration is regulated by buffer, alpha-Ltx also enhances Ca2+-dependent secretion, indicating a direct role of the endogenous receptor in the secretory pathway. Expression of CIRL increased the sensitivity of intact and permeabilized cells to the effects of alpha-Ltx, demonstrating that this protein is functional in coupling to secretion. Importantly, in the absence of alpha-Ltx, the expression of CIRL specifically inhibited the ATP-dependent component of secretion in permeabilized cells without affecting the ATP-independent secretion. This suggests that this receptor modulates the normal function of the regulated secretory pathway and that alpha-Ltx may act by reversing the inhibitory effects of the receptor. |
| | |
Authors:
|
M A Bittner; V G Krasnoperov; E L Stuenkel; A G Petrenko; R W Holz |
Related Documents
:
|
18369948 - Molecular dissection of hcl secretion in gastric parietal cells using streptolysin o pe... 2468678 - Effects of n-alcohols on junctional coupling and amylase secretion of pancreatic acinar... 9846648 - Examination of somatostatin involvement in the inhibitory action of gip, glp-1, amylin ... 7875488 - Secretin causes h+/hco3- secretion from pig pancreatic ductules by vacuolar-type h(+)-a... 8226518 - Importance of prostanoids in calcium chelator-induced airway constriction. 16985238 - Simultaneous optical measurements of cytosolic ca2+ and camp in single cells. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The Journal of neuroscience : the official journal of the Society for Neuroscience Volume: 18 ISSN: 0270-6474 ISO Abbreviation: J. Neurosci. Publication Date: 1998 Apr |
Date Detail:
|
Created Date: 1998-04-20 Completed Date: 1998-04-20 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 8102140 Medline TA: J Neurosci Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 2914-22 Citation Subset: IM |
Affiliation:
|
Department of Pharmacology, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenosine Triphosphate
/
pharmacology Animals Calcium / pharmacokinetics Catecholamines / secretion Cattle Cell Membrane Permeability / drug effects Cells, Cultured Chelating Agents / pharmacology Chromaffin Cells / chemistry*, cytology, secretion* Digitonin Egtazic Acid / pharmacology Exocytosis / drug effects, physiology Gene Expression Humans Kidney / cytology Kinetics Nerve Tissue Proteins / genetics, metabolism Receptors, Peptide / genetics, metabolism* Spider Venoms / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
|
R01DK27959/DK/NIDDK NIH HHS; R01NS34937/NS/NINDS NIH HHS; R01NS35098/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Catecholamines; 0/Chelating Agents; 0/Nerve Tissue Proteins; 0/Receptors, Peptide; 0/Spider Venoms; 0/alpha-latrotoxin receptor; 11024-24-1/Digitonin; 56-65-5/Adenosine Triphosphate; 65988-34-3/alpha-latrotoxin; 67-42-5/Egtazic Acid; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Protein kinase C activation increases release of secreted amyloid precursor protein without decreasi...
Next Document: A neuronal Sec1 homolog regulates neurotransmitter release at the squid giant synapse.