Document Detail

Ca2+-dependent protein phosphorylation associated with microsomal fraction of rat pancreas.
MedLine Citation:
PMID:  3743879     Owner:  NLM     Status:  MEDLINE    
Microsomes isolated from cat pancreas were incubated with [gamma-32P]ATP in the presence or absence of Ca2+. Following fractionation of phosphoproteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis a single microsomal protein with an apparent molecular mass of 77,000 dalton (77K) was found to be phosphorylated in a Ca2+-dependent mechanism. Maximal phosphate incorporation into the 77K protein was observed at 10(-6) mol/l [Ca2+] and was 4-fold higher than in the absence of Ca2+. The 77K phosphoprotein showed characteristic of a stable phosphoester rather than an acyl phosphate. Measurable phosphate incorporation into the 77K protein was noted 5 s following addition of [gamma-32P]ATP and reached maximum at 9-10th min. The lack of effect of exogenous cyclic AMP, cyclic AMP-dependent protein kinase, calmodulin, the calmodulin antagonist trifluoperazine, leupeptin and the suppression of phosphorylation by some phospholipid-interacting drugs suggested that the 77K protein is a substrate for cyclic AMP- and calmodulin-independent, Ca2+-activated phospholipid-sensitive kinase activity. Centrifugation of the pancreatic homogenate in a ficoll-sucrose density gradient indicated that both the 77K protein and enzyme were associated in a fraction enriched in rough endoplasmic reticulum.
A C Shisheva; K Imamura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The International journal of biochemistry     Volume:  18     ISSN:  0020-711X     ISO Abbreviation:  Int. J. Biochem.     Publication Date:  1986  
Date Detail:
Created Date:  1986-10-22     Completed Date:  1986-10-22     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0250365     Medline TA:  Int J Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  753-61     Citation Subset:  IM    
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MeSH Terms
Calcium / pharmacology*
Microsomes / metabolism*
Molecular Weight
Pancreas / metabolism*
Phosphoproteins / isolation & purification*
Phosphorus Radioisotopes
Proteins / metabolism*
Reg. No./Substance:
0/Phosphoproteins; 0/Phosphorus Radioisotopes; 0/Proteins; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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