Document Detail

A Ca(2+)-dependent phosphorylcholine-binding protein in chicken serum.
MedLine Citation:
PMID:  8286541     Owner:  NLM     Status:  MEDLINE    
A Ca(2+)-dependent phosphorylcholine (PC)-binding protein in normal chicken serum was purified by affinity chromatography on p-aminophenyl PC-Sepharose 4B followed by gel filtration on Sephacryl S-300. In gel filtration, the isolated PC-binding protein was eluted in a symmetrical protein peak at the position of approximately 100,000. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the protein was resolved into two protein bands of 31,000 and 38,000 under nonreducing conditions and of 40,000 and 46,000 under reducing conditions. These results suggest that chicken serum PC-binding protein may be composed of two different subunits which contain intrachain disulfide bonds.
S Sugii; Y Hirota
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of veterinary medical science / the Japanese Society of Veterinary Science     Volume:  55     ISSN:  0916-7250     ISO Abbreviation:  J. Vet. Med. Sci.     Publication Date:  1993 Oct 
Date Detail:
Created Date:  1994-02-22     Completed Date:  1994-02-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9105360     Medline TA:  J Vet Med Sci     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  841-3     Citation Subset:  IM    
Department of Serology and Immunology, School of Medical Technology, Kitasato University, Kanagawa, Japan.
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MeSH Terms
Calcium / metabolism*
Carrier Proteins / blood*,  isolation & purification
Chickens / blood*
Chromatography, Affinity
Chromatography, Gel
Electrophoresis, Polyacrylamide Gel
Molecular Weight
Phosphorylcholine / metabolism
Reg. No./Substance:
0/Carrier Proteins; 107-73-3/Phosphorylcholine; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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