| Ca2+-dependent induction of intracellular Ca2+ oscillation in hippocampal astrocytes during metabotropic glutamate receptor activation. | |
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MedLine Citation:
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PMID: 15684567 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have investigated whether the intracellular calcium concentration ([Ca(2+)](i)) oscillations induced in astrocytes using the metabotropic glutamate-receptor agonist, (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (t-ACPD) are Ca(2+)-dependent, using three different Ca(2+) indicators with different affinities for Ca(2+). When rat hippocampal cells in culture were loaded with fura-2 (K(d): 145 nM), two-thirds of the cells showed obvious oscillatory increase in [Ca (2+)](i) during t-ACPD-administration. Those cells were identified as astrocytes by immuno-histochemistry in our previous paper. In cells loaded with fura-2FF (K(d): 25,000 nM), a similar percentage of t-ACPD-responsive cells showed oscillatory [Ca(2+)](i) changes. However, in cells loaded with quin-2 (K(d): 60 nM), t-ACPD induced no oscillatory responses, but some cells showed a small transient increase in the [Ca(2+)](i). The same small transient [Ca(2+)](i) increase was seen in cells loaded with both fura-2FF and BAPTA, a Ca(2+) chelator (K(d): 135 nM). These findings indicate the involvement of [Ca(2+)](i)-dependent regulatory mechanisms in the induction of the t-ACPD-induced oscillatory change in the [Ca(2+)](i) in astrocytes. |
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Authors:
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Yoshitoku Yoshida; Remi Tsuchiya; Nagisa Matsumoto; Mitsuhiro Morita; Hiroyoshi Miyakawa; Yoshihisa Kudo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-01-30 |
Journal Detail:
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Title: Journal of pharmacological sciences Volume: 97 ISSN: 1347-8613 ISO Abbreviation: J. Pharmacol. Sci. Publication Date: 2005 Feb |
Date Detail:
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Created Date: 2005-02-28 Completed Date: 2005-08-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 101167001 Medline TA: J Pharmacol Sci Country: Japan |
Other Details:
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Languages: eng Pagination: 212-8 Citation Subset: IM |
Affiliation:
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Molecular Life Science Division, School of Life Science, Tokyo University of Pharmacy and Life Science, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Astrocytes / drug effects, metabolism*, physiology Calcium / physiology* Calcium Signaling / drug effects, physiology* Cells, Cultured Cycloleucine / analogs & derivatives, pharmacology Female Hippocampus / drug effects, metabolism*, physiology Intracellular Fluid / metabolism*, physiology Pregnancy Rats Rats, Wistar Receptors, Metabotropic Glutamate / agonists, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Metabotropic Glutamate; 111900-32-4/1-amino-1,3-dicarboxycyclopentane; 52-52-8/Cycloleucine; 7440-70-2/Calcium |
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