Document Detail


Ca2+-dependent induction of intracellular Ca2+ oscillation in hippocampal astrocytes during metabotropic glutamate receptor activation.
MedLine Citation:
PMID:  15684567     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have investigated whether the intracellular calcium concentration ([Ca(2+)](i)) oscillations induced in astrocytes using the metabotropic glutamate-receptor agonist, (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (t-ACPD) are Ca(2+)-dependent, using three different Ca(2+) indicators with different affinities for Ca(2+). When rat hippocampal cells in culture were loaded with fura-2 (K(d): 145 nM), two-thirds of the cells showed obvious oscillatory increase in [Ca (2+)](i) during t-ACPD-administration. Those cells were identified as astrocytes by immuno-histochemistry in our previous paper. In cells loaded with fura-2FF (K(d): 25,000 nM), a similar percentage of t-ACPD-responsive cells showed oscillatory [Ca(2+)](i) changes. However, in cells loaded with quin-2 (K(d): 60 nM), t-ACPD induced no oscillatory responses, but some cells showed a small transient increase in the [Ca(2+)](i). The same small transient [Ca(2+)](i) increase was seen in cells loaded with both fura-2FF and BAPTA, a Ca(2+) chelator (K(d): 135 nM). These findings indicate the involvement of [Ca(2+)](i)-dependent regulatory mechanisms in the induction of the t-ACPD-induced oscillatory change in the [Ca(2+)](i) in astrocytes.
Authors:
Yoshitoku Yoshida; Remi Tsuchiya; Nagisa Matsumoto; Mitsuhiro Morita; Hiroyoshi Miyakawa; Yoshihisa Kudo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-01-30
Journal Detail:
Title:  Journal of pharmacological sciences     Volume:  97     ISSN:  1347-8613     ISO Abbreviation:  J. Pharmacol. Sci.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-02-28     Completed Date:  2005-08-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101167001     Medline TA:  J Pharmacol Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  212-8     Citation Subset:  IM    
Affiliation:
Molecular Life Science Division, School of Life Science, Tokyo University of Pharmacy and Life Science, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Astrocytes / drug effects,  metabolism*,  physiology
Calcium / physiology*
Calcium Signaling / drug effects,  physiology*
Cells, Cultured
Cycloleucine / analogs & derivatives,  pharmacology
Female
Hippocampus / drug effects,  metabolism*,  physiology
Intracellular Fluid / metabolism*,  physiology
Pregnancy
Rats
Rats, Wistar
Receptors, Metabotropic Glutamate / agonists,  metabolism*
Chemical
Reg. No./Substance:
0/Receptors, Metabotropic Glutamate; 111900-32-4/1-amino-1,3-dicarboxycyclopentane; 52-52-8/Cycloleucine; 7440-70-2/Calcium

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