| Ca2+, Mg2+ and K+ transport in the cortical and medullary thick ascending limb of the rat nephron: influence of transepithelial voltage. | |
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MedLine Citation:
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PMID: 8255743 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Isolated segments of rat cortical (cTAL) and medullary (mTAL) thick ascending limbs were microperfused and the transepithelial net fluxes (JX) were determined by measuring the composition of the collected fluid with an electron microprobe. When perfused with symmetrical solutions both segments showed similar JNa and JCl and lumen-positive transepithelial voltage (Vte = 7-8 mV). JMg, JCa and JK were not significantly different from zero. When perfused with asymmetrical solutions (lumen 50 mM, bath 150 mM NaCl), the mean Vte were 23 mV and 17 mV in the cTAL and mTAL respectively; this rise was accompanied by significant increases in JMg and JCa in the cTAL, but not in the mTAL, and a marked increase in JK in both segments. It is concluded that, in the rat, divalent cations can be reabsorbed in the cTAL, and K+ can be reabsorbed in the cTAL and mTAL. The transport is voltage-dependent. The mTAL can reabsorb neither Mg2+ nor Ca2+, whatever Vte. |
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Authors:
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B Mandon; E Siga; N Roinel; C de Rouffignac |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Pflügers Archiv : European journal of physiology Volume: 424 ISSN: 0031-6768 ISO Abbreviation: Pflugers Arch. Publication Date: 1993 Sep |
Date Detail:
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Created Date: 1994-01-13 Completed Date: 1994-01-13 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0154720 Medline TA: Pflugers Arch Country: GERMANY |
Other Details:
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Languages: eng Pagination: 558-60 Citation Subset: IM |
Affiliation:
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Département de Biologie Cellulaire et Moléculaire, CEA-Saclay, Gif-Sur-Yvette, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport Calcium / metabolism* Electric Stimulation Epithelium / physiology Female Kidney Cortex / metabolism* Kidney Medulla / metabolism* Magnesium / metabolism* Nephrons / metabolism* Potassium / metabolism* Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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7439-95-4/Magnesium; 7440-09-7/Potassium; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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