Document Detail


CYP2B1 is regulated by C/EBP alpha and C/EBP delta inlung epithelial cells.
MedLine Citation:
PMID:  10683316     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pulmonary expression of several cytochrome P450 (CYP) monooxygenases is detected late in gestation. Little is known of the factors involved in this differentiation-dependent expression. C/EBP factors are known regulators of differentiation and differentiation-dependent gene expression in several tissues. In this study we demonstrate the importance of C/EBP alpha and C/EBP delta in pulmonary epithelial CYP2B1 gene expression. A 1.3 kb CYP2B1 promoter fragment which recently has been shown to confer lung tissue- and cell-specific expression of CYP2B1 in transgenic mice was used in transient transfection studies. Both C/EBP alpha and C/EBP delta transactivated the CYP2B1 promoter in the lung epithelial cell lines A549 and NCI-H441. C/EBP alpha in nuclear extracts from isolated rat primary bronchiolar Clara cells was capable of interacting with a C/EBP-binding site in the proximal CYP2B1 promoter. Site-directed mutagenesis studies showed that this proximal C/EBP-binding site is necessary for transactivation of the CYP2B1 gene by C/EBP alpha and C/EBP delta. Thi study shows that C/EBP factors have a role in pulmonary CYP2B1 expression and suggests that these transcription factors may be important for the differentiation-dependent expression of CYP2B1 in the lung.
Authors:
T N Cassel; J A Gustafsson; M Nord
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular cell biology research communications : MCBRC     Volume:  3     ISSN:  1522-4724     ISO Abbreviation:  Mol. Cell Biol. Res. Commun.     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-09-15     Completed Date:  2000-09-15     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100889076     Medline TA:  Mol Cell Biol Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  42-7     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Academic Press.
Affiliation:
Department of Medical Nutrition, Karolinska Institutet, Huddinge University Hospital, F60 Novum, Huddinge, S-141 86, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Animals
Binding Sites
Blotting, Western
Bronchi / cytology,  enzymology
CCAAT-Enhancer-Binding Protein-delta
CCAAT-Enhancer-Binding Proteins*
COS Cells
Cell Line
Cells, Cultured
Cytochrome P-450 CYP2B1 / metabolism*
DNA-Binding Proteins / metabolism*
Electrophoresis, Polyacrylamide Gel
Epithelial Cells / enzymology*
Gene Expression Regulation, Developmental*
Humans
Lung / enzymology*
Mutagenesis, Site-Directed
Nuclear Proteins / metabolism*
Plasmids / metabolism
Promoter Regions, Genetic
Rats
Transcription Factors*
Transcriptional Activation
Transfection
Chemical
Reg. No./Substance:
0/CCAAT-Enhancer-Binding Proteins; 0/CEBPD protein, human; 0/Cebpd protein, rat; 0/DNA-Binding Proteins; 0/Nuclear Proteins; 0/Transcription Factors; 142662-43-9/CCAAT-Enhancer-Binding Protein-delta; EC 1.14.14.1/Cytochrome P-450 CYP2B1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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