Document Detail


CXCR-4 knockdown by small interfering RNA inhibits cell proliferation and invasion of oral squamous cell carcinoma cells.
MedLine Citation:
PMID:  18624931     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Oral squamous cell carcinomas (OSCCs) are characterized by a high degree of local invasion and a high rate of metastases to cervical lymph nodes. Downregulation of CXCR-4 by siRNA inhibits invasion and growth of breast and colon cancer cells. However, there have been no reports on the downregulation of CXCR-4 by small interfering RNA (siRNA) in oral cancer cells. METHODS: We generated two stable CXCR-4-knockdown clones (KBsi and KOSCC-25Bsi) from the KB and KOSCC-25B OSCC cell lines by lentiviral delivery. In vitro invasion and cell proliferation assays were used to investigate the effect of CXCR-4 downregulation on cell proliferation and invasiveness in KBsi and KOSCC-25Bsi. Immunohistochemistry was performed to evaluate the correlation between CXCR-4 expression and proliferation in 26 OSCC tissue samples. RESULTS: CXCR4-knockdown OSCC cells showed reduced invasiveness. The invasiveness of KBsi decreased to 29.5% of the vector-infected controls, and KOSCC-25Bsi decreased to 38.1% of the control vector-infected cells (P < 0.05). The CXCR4-knockdown OSCC cells grew significantly slower than the vector-infected control cells. KBsi and KOSCC-25Bsi cells proliferated at 69.5% and 71.7%, respectively, of the rate of control vector-infected cells (P < 0.05). CXCR-4-positive group had significantly higher PCNA labeling index than CXCR-4-negative group in OSCC tissue samples. CONCLUSION: These results suggest that the downregulation of CXCR-4 induces anti-proliferative and anti-invasive effects in OSCC and that CXCR-4 might be a useful target molecule for the treatment of OSCC.
Authors:
Ji-Soo Hong; Hyun-Kyung Pai; Kyoung-Ok Hong; Mi-Ae Kim; Ji-Hong Kim; Jae-Il Lee; Sam-Pyo Hong; Seong-Doo Hong
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-12
Journal Detail:
Title:  Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology     Volume:  38     ISSN:  1600-0714     ISO Abbreviation:  J. Oral Pathol. Med.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-02-09     Completed Date:  2009-05-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8911934     Medline TA:  J Oral Pathol Med     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  214-9     Citation Subset:  D; IM    
Affiliation:
Department of Oral Pathology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Carcinoma, Squamous Cell / genetics*
Cell Line, Tumor
Cell Proliferation
Clone Cells
Down-Regulation
Gene Expression Regulation, Neoplastic
Gene Knockdown Techniques
Humans
Immunoblotting
Lymphatic Metastasis / genetics
Mouth Neoplasms / genetics*
Neoplasm Invasiveness / genetics
Proliferating Cell Nuclear Antigen / analysis
RNA, Small Interfering / physiology
Receptors, CXCR4 / biosynthesis,  genetics*,  physiology*
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/CXCR4 protein, human; 0/Proliferating Cell Nuclear Antigen; 0/RNA, Small Interfering; 0/Receptors, CXCR4

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