Document Detail

A CTX family cell adhesion molecule, JAM4, is expressed in stem cell and progenitor cell populations of both male germ cell and hematopoietic cell lineages.
MedLine Citation:
PMID:  16982697     Owner:  NLM     Status:  MEDLINE    
Stem cells are maintained in an undifferentiated state by interacting with a microenvironment known as the "niche," which is comprised of various secreted and membrane proteins. Our goal was to identify niche molecules participating in stem cell-stem cell and/or stem cell-supporting cell interactions. Here, we isolated genes encoding secreted and membrane proteins from purified male germ stem cells using a signal sequence trap approach. Among the genes identified, we focused on the junctional adhesion molecule 4 (JAM4), an immunoglobulin type cell adhesion molecule. JAM4 protein was actually localized to the plasma membrane in male germ cells. JAM4 expression was downregulated as cells differentiated in both germ cell and hematopoietic cell lineages. To analyze function in vivo, we generated JAM4-deficient mice. Histological analysis of testes from homozygous nulls did not show obvious abnormalities, nor did liver and kidney tissues, both of which strongly express JAM4. The numbers of hematopoietic stem cells in bone marrow were indistinguishable between wild-type and mutant mice, as was male germ cell development. These results suggest that JAM4 is expressed in stem cells and progenitor cells but that other cell adhesion molecules may substitute for JAM4 function in JAM4-deficient mice both in male germ cell and hematopoietic lineages.
Go Nagamatsu; Masako Ohmura; Takuo Mizukami; Isao Hamaguchi; Susumu Hirabayashi; Shosei Yoshida; Yutaka Hata; Toshio Suda; Kazuyuki Ohbo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-09-18
Journal Detail:
Title:  Molecular and cellular biology     Volume:  26     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-11-01     Completed Date:  2007-01-09     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8498-506     Citation Subset:  IM    
Sakaguchi Laboratory, Department of Cell Differentiation, School of Medicine, Keio University, Tokyo, Japan.
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MeSH Terms
Animals, Newborn
Cell Adhesion
Cell Adhesion Molecules / genetics,  isolation & purification,  metabolism*
Cell Differentiation
Germ Cells / metabolism*
Hematopoietic Stem Cells / metabolism*,  physiology
Kidney / metabolism
Liver / metabolism
Mice, Inbred C57BL
Mice, Inbred ICR
Mice, Knockout
Stem Cells / metabolism*
Testis / cytology,  metabolism
Reg. No./Substance:
0/Cell Adhesion Molecules; 0/Igsf5 protein, mouse

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