Document Detail


CT406 encodes a chlamydial ortholog of NrdR, a repressor of ribonucleotide reductase.
MedLine Citation:
PMID:  21725017     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chlamydia trachomatis is an obligate intracellular bacterium that is dependent on its host cell for nucleotides. Chlamydia imports ribonucleotide triphosphates (NTPs) but not deoxyribonucleotide triphosphates (dNTPs) and instead uses ribonucleotide reductase to convert imported ribonucleotides into deoxyribonucleotides for DNA synthesis. The genes encoding ribonucleotide reductase have been recently shown to be negatively controlled by a conserved regulator called NrdR. In this study, we provide direct evidence that Escherichia coli NrdR is a transcriptional repressor and that C. trachomatis CT406 encodes its chlamydial ortholog. We showed that CT406 binds specifically to two NrdR boxes upstream of the nrdAB operon in C. trachomatis. Using an in vitro transcription assay, we confirmed that these NrdR boxes function as an operator since they were necessary and sufficient for CT406-mediated repression. We validated our in vitro findings with reporter studies in E. coli showing that both E. coli NrdR and CT406 repressed transcription from the E. coli nrdH and C. trachomatis nrdAB promoters in vivo. This in vivo repression was reversed by hydroxyurea treatment. Since hydroxyurea inhibits ribonucleotide reductase and reduces intracellular deoxyribonucleotide levels, these results suggest that NrdR activity is modulated by a deoxyribonucleotide corepressor.
Authors:
Elizabeth Di Russo Case; Johnny C Akers; Ming Tan
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-07-01
Journal Detail:
Title:  Journal of bacteriology     Volume:  193     ISSN:  1098-5530     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-08-12     Completed Date:  2011-10-06     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4396-404     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics,  metabolism*
Base Sequence
Chlamydia trachomatis / genetics*,  metabolism
Chromosome Mapping
DNA Replication
DNA, Bacterial
Deoxyribonucleotides / analysis,  metabolism
Escherichia coli / genetics
Escherichia coli Proteins / genetics,  metabolism
Gene Expression Regulation, Bacterial
Genes, Bacterial
Hydroxyurea / pharmacology
Molecular Sequence Data
Operon
Plasmids
Repressor Proteins / genetics,  metabolism*
Ribonucleotide Reductases / genetics*,  metabolism
Transcription, Genetic
Grant Support
ID/Acronym/Agency:
AI 057563/AI/NIAID NIH HHS; AI 44198/AI/NIAID NIH HHS; K02 AI057563/AI/NIAID NIH HHS; R01 AI044198/AI/NIAID NIH HHS; R56 AI044198/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA, Bacterial; 0/Deoxyribonucleotides; 0/Escherichia coli Proteins; 0/NrdR protein, E coli; 0/Repressor Proteins; EC 1.17.4.-/Ribonucleotide Reductases; X6Q56QN5QC/Hydroxyurea
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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