Document Detail

CREG promotes a mature smooth muscle cell phenotype and reduces neointimal formation in balloon-injured rat carotid artery.
MedLine Citation:
PMID:  18267954     Owner:  NLM     Status:  MEDLINE    
AIM: We previously showed that cellular repressor of E1A-stimulated genes (CREG) is up-regulated during serum starvation-induced vascular smooth muscle cell (SMC) differentiation. The aim of this study was to determine the role of CREG in maintaining the quiescent, differentiated phenotype of SMCs both in culture and in balloon-injured rat carotid artery. METHODS AND RESULTS: In cultured SMCs recombinant virus-mediated CREG expression enhanced cellular differentiation, inhibited proliferation, and reduced synthesis of extracellular matrix component fibronectin. In contrast, CREG knockdown via retroviral transfer of short hairpin RNAs abrogated serum starvation-induced SMC differentiation and growth arrest. Both immunostaining and Western analysis demonstrated marked down-regulation of CREG in the vascular media after balloon injury to the rat carotid artery. Retrovirus-mediated CREG transfer to the injured artery inhibited SMC dedifferentiation and proliferation, and reduced neointimal hyperplasia. CONCLUSION: These results suggest that CREG participates in the maintenance of quiescent mature SMC phenotype in the arterial media by promoting SMC differentiation and growth arrest and that CREG gene transfer has therapeutic potential for vascular diseases associated with neointimal hyperplasia.
Yaling Han; Jie Deng; Liang Guo; Chenghui Yan; Ming Liang; Jian Kang; Haiwei Liu; Alan M Graham; Shaohua Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-11
Journal Detail:
Title:  Cardiovascular research     Volume:  78     ISSN:  0008-6363     ISO Abbreviation:  Cardiovasc. Res.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-20     Completed Date:  2008-09-09     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0077427     Medline TA:  Cardiovasc Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  597-604     Citation Subset:  IM    
Cardiovascular Research Institute and Department of Cardiology, Shenyang Northern Hospital, 83 Wenhua Road, Shenyang 110016, People's Republic of China.
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MeSH Terms
Balloon Dilatation / adverse effects
Blotting, Western
Carotid Artery Injuries / etiology,  metabolism*,  pathology
Cell Differentiation
Cell Proliferation
Cells, Cultured
Culture Media, Serum-Free
Enzyme Activation
Extracellular Signal-Regulated MAP Kinases / metabolism
Fibronectins / metabolism
Gene Transfer Techniques
Genetic Vectors
Muscle, Smooth, Vascular / enzymology,  metabolism*,  pathology
Myocytes, Smooth Muscle / enzymology,  metabolism*,  pathology
RNA Interference
Rats, Sprague-Dawley
Repressor Proteins / genetics,  metabolism*
Retroviridae / genetics
Time Factors
Tunica Intima / metabolism*,  pathology
Reg. No./Substance:
0/CREG1 protein, human; 0/Culture Media, Serum-Free; 0/Fibronectins; 0/Repressor Proteins; EC Signal-Regulated MAP Kinases

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