| CRABP I expression and the mediation of the sensitivity of human tumour cells to retinoic acid and irradiation. | |
| | |
MedLine Citation:
|
PMID: 14713576 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
PURPOSE: To examine the role cytoplasmic retinoic acid binding protein type 1 (CRABP I) and retinoic acid receptor beta 2 (RAR-beta 2) in mediating radiosensitization of human tumour cells in vitro by retinoic acid. MATERIALS AND METHODS: Human squamous cell carcinoma cell lines of different types were treated with retinoic acid followed by irradiation. Radiation response under drug treatment was detected by colony-formation assay. mRNA and protein expression levels of CRABP I, RAR-beta and cyclin D1 were investigated under different treatment conditions by room temperature polymerase chain reaction and Western blotting. The retinoic acid-sensitive cell line HTB35 was transfected for inducible CRABP I overexpression to test the role of this protein in modulating the sensitivity to retinoic acid and radiation as well as in regulating RAR-beta 2 and cyclin D1 expression. RESULTS: The basal CRABP I level clearly correlated with the clonogenic survival of tumour cells and normal fibroblasts after treatment with retinoic acid and ionizing irradiation (IR). Cells expressing high basal CRABP I were more resistant to combined retinoic acid radiation treatment than cells with low basal expression. Overexpression of CRABP I in retinoic acid-sensitive HTB35 cells induced a retinoic acid-insensitive phenotype resistant to combined treatment with retinoic acid and radiation. This effect was independent of RAR-beta 2 expression. CRABP I overexpression resulted in stimulated cyclin D1 expression indicating the dependency of this cell cycle control protein on retinoic acid metabolism. CONCLUSION: CRABP I plays an important role not only in mediating the retinoid effects, but also in modulating the radiation sensitivity of tumour cells after combined retinoic acid radiation treatment. |
| | |
Authors:
|
M A Blaese; L Santo-Hoeltje; H P Rodemann |
Related Documents
:
|
1988666 - Bmy 30047: a novel topically active retinoid with low local and systemic toxicity. 8218526 - Determination of the kinetics of degradation of 13-cis-retinoic acid and all-trans-reti... 23536 - Antagonism of histamine-activated adenylate cyclase in brain by d-lysergic acid diethyl... 8674046 - Accelerated degradation of pml-retinoic acid receptor alpha (pml-rara) oncoprotein by a... 447976 - Influence of oral contraceptives on ascorbic acid and triglyceride status. 3942006 - Short-term effects of various phenols and acids on the fischer 344 male rat forestomach... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: International journal of radiation biology Volume: 79 ISSN: 0955-3002 ISO Abbreviation: Int. J. Radiat. Biol. Publication Date: 2003 Dec |
Date Detail:
|
Created Date: 2004-01-09 Completed Date: 2004-04-06 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8809243 Medline TA: Int J Radiat Biol Country: England |
Other Details:
|
Languages: eng Pagination: 981-91 Citation Subset: IM; S |
Affiliation:
|
Section of Radiobiology and Molecular Environmental Research, Eberhard-Karls-University Tuebingen, Roentgenweg 11, D-72076 Tuebingen, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Line, Tumor
/
metabolism,
pathology,
radiation effects Cell Survival / drug effects, radiation effects Dose-Response Relationship, Drug Dose-Response Relationship, Radiation Fibroblasts / cytology, drug effects*, metabolism, radiation effects* Humans Neoplasms / metabolism*, pathology* Radiation Tolerance / drug effects* Radiation-Sensitizing Agents / pharmacology Receptors, Retinoic Acid / metabolism* Tretinoin / pharmacology* Tumor Stem Cell Assay |
| Chemical | |
Reg. No./Substance:
|
0/Radiation-Sensitizing Agents; 0/Receptors, Retinoic Acid; 0/retinoic acid binding protein I, cellular; 0/retinoic acid receptor beta; 302-79-4/Tretinoin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Geldanamycin, an inhibitor of Hsp90, sensitizes human tumour cells to radiation.
Next Document: Low-dose radiotherapy (LD-RT) and the modulation of iNOS expression in adjuvant-induced arthritis in...