Document Detail


COX-2 Inhibition by Use of Rofecoxib or High Dose Aspirin Enhances ADP-Induced Platelet Aggregation in Fresh Blood.
MedLine Citation:
PMID:  21331307     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
AIM: Increased cardiovascular risk after use of selective or nonselective cyclooxygenase-2 (COX-2)-inhibitors might partly be caused by enhanced platelet aggregability. However, an effect of COX-2 inhibition on platelets has so far not been observed in humans.
METHODS: We tested in healthy volunteers the effect of COX-2-inhibition nearly in-vivo, i.e. immediately after and even during blood sampling.
RESULTS: Measurement within 2 minutes after venipuncture, but not 60 minutes later, showed that 50 mg of rofecoxib (n=12) or 500 (n=8) or 1000 (n=8) mg of aspirin increased ADP-induced platelet aggregation in a whole-blood aggregometer to, respectively, 152, 176 and 204 % of basal level (p<0.01). No significant differences in aggregability were observed after ingestion of 80 mg of aspirin (n=16), or placebo (n=8). Plasma 6-keto-PGF1α was decreased to 74 % after rofecoxib and to 76 and 70 % after 500 and 1000 mg of aspirin but did not change after low dose aspirin. Continuous photometrical measurement of aggregation in blood flowing from a cannulated vein revealed that high dose aspirin did not elicit aggregation by itself, but increased ADP-induced aggregation in proportion to the decrease in prostacyclin formation (r=0.68, p = 0.004). Since in these experiments thromboxane production was virtually absent, the enhanced aggregation after partial COX-2 inhibition was not caused by unopposed thromboxane formation.
CONCLUSIONS: We conclude that both selective and nonselective COX-2 inhibition enhances ADP-induced platelet aggregation in humans. This effect can only be detected during or immediately after venipuncture, possibly because of the short half-life of prostacyclin.
Authors:
Piet Borgdorff; M Louis Handoko; Yeun Ying Wong; Geert Jan Tangelder
Related Documents :
3420667 - Experimental basis for the use of red cell transfusion in the management of anemic-thro...
22264757 - Substrate specificity of acetoxy derivatives of coumarins and quinolones towards calret...
15994287 - Response criteria for myelofibrosis with myeloid metaplasia: results of an initiative o...
635007 - Platelet estimation in whole blood.
1190507 - Stimulation of sperm motility by kinins in fresh and 24 hours aged human ejaculates.
1574877 - Heparin-induced thrombocytopenia with thrombotic complications.
Publication Detail:
Type:  Journal Article     Date:  2010-10-21
Journal Detail:
Title:  The open cardiovascular medicine journal     Volume:  4     ISSN:  1874-1924     ISO Abbreviation:  Open Cardiovasc Med J     Publication Date:  2010  
Date Detail:
Created Date:  2011-02-18     Completed Date:  2011-07-14     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  101480504     Medline TA:  Open Cardiovasc Med J     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  198-205     Citation Subset:  -    
Affiliation:
Institute for Cardiovascular Research, Vrije Universiteit Medical Center, Amsterdam, The Netherlands.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Destructive dural ectasia of dorsal and lumbar spine with cauda equina syndrome in a patient with an...
Next Document:  High throughput screening for inhibitors of alpha-galactosidase.