Document Detail

COG6 interacts with a subset of the Golgi SNAREs and is important for the Golgi complex integrity.
MedLine Citation:
PMID:  23057818     Owner:  NLM     Status:  MEDLINE    
Vesicular tethers and SNAREs are two key protein components that govern docking and fusion of intracellular membrane carriers in eukaryotic cells. The conserved oligomeric Golgi (COG) complex has been specifically implicated in the tethering of retrograde intra-Golgi vesicles. Using yeast two-hybrid and co-immunoprecipitation approaches, we show that the COG6 subunit of the COG complex is capable of interacting with a subset of Golgi SNAREs, namely STX5, STX6, GS27 and SNAP29. Interaction with SNAREs is accomplished via the universal SNARE-binding motif of COG6. Overexpression of COG6, or its depletion from cells, disrupts the integrity of the Golgi complex. Importantly, COG6 protein lacking the SNARE-binding domain is deficient in Golgi binding, and is not capable of inducing Golgi complex fragmentation when overexpressed. These results indicate that COG6-SNARE interactions are important for both COG6 localization and Golgi integrity.
Tetyana Kudlyk; Rose Willett; Irina D Pokrovskaya; Vladimir Lupashin
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.     Date:  2012-11-12
Journal Detail:
Title:  Traffic (Copenhagen, Denmark)     Volume:  14     ISSN:  1600-0854     ISO Abbreviation:  Traffic     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-07     Completed Date:  2013-06-11     Revised Date:  2014-02-04    
Medline Journal Info:
Nlm Unique ID:  100939340     Medline TA:  Traffic     Country:  England    
Other Details:
Languages:  eng     Pagination:  194-204     Citation Subset:  IM    
Copyright Information:
© 2012 John Wiley & Sons A/S.
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MeSH Terms
Adaptor Proteins, Vesicular Transport / genetics,  metabolism*
Binding Sites
Golgi Apparatus / metabolism*
HeLa Cells
Protein Binding
RNA, Small Interfering
SNARE Proteins / genetics,  metabolism*
Transport Vesicles / metabolism
Two-Hybrid System Techniques
Grant Support
Reg. No./Substance:
0/Adaptor Proteins, Vesicular Transport; 0/COG6 protein, human; 0/RNA, Small Interfering; 0/SNARE Proteins

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