| CO2 modulates the inflammatory cytokine release of primary human pleural macrophages. | |
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MedLine Citation:
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PMID: 20397120 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: It is well known that CO (2) used during laparoscopy affects the peritoneal surface and local inflammatory response, including the inflammatory reactivity of peritoneal macrophages. However, little is known about the local effects of CO (2) during thoracoscopy. In a previous study we have shown that in healthy adolescents, macrophages are the dominant cell population on the pleural surface. Therefore, we examined the effects of CO (2) on the inflammatory response of primary human pleural macrophages. METHODS: Human primary macrophages were harvested lavage from healthy adolescents undergoing elective surgery for pectus bar correction (n=8). After purification and 24 h resting, cells were incubated for 2 h in 100% CO (2), 5% CO (2) or 95% inert helium with 5% CO (2) as hypoxic control. After incubation cells were stimulated with LPS for 4 h and 24 h. The release of TNF-alpha, IL-8, IL-6, IL-10 and IL-1 beta were determined by ELISA. RESULTS: CO (2), but not hypoxia, induced a significant reduction in the release of TNF-alpha and IL-8 as well as a significant increase in the release of IL-10 and IL-1 beta within the first 4 h after incubation. The levels of IL-6 and the release of cytokines at 24 h after incubation were not significantly affected. CONCLUSIONS: CO (2) directly modulates the immediate inflammatory response of pleural macrophages. Therefore, CO (2) insufflation during thoracoscopy could lower the local stress response, but does not appear to have a lasting effect. |
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Authors:
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A Shimotakahara; M Metzelder; G Vieten; B Ure; J F Kuebler |
Publication Detail:
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Type: Journal Article Date: 2010-04-15 |
Journal Detail:
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Title: European journal of pediatric surgery : official journal of Austrian Association of Pediatric Surgery ... [et al] = Zeitschrift für Kinderchirurgie Volume: 20 ISSN: 1439-359X ISO Abbreviation: Eur J Pediatr Surg Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-04-16 Completed Date: 2010-09-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9105263 Medline TA: Eur J Pediatr Surg Country: Germany |
Other Details:
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Languages: eng Pagination: 111-5 Citation Subset: IM |
Affiliation:
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Jutendo University, Pediatric Surgery, Tokyo, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Carbon Dioxide / metabolism* Cytokines / metabolism* Female Humans Inflammation / metabolism* Macrophages / metabolism* Male Pleura / cytology* |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 124-38-9/Carbon Dioxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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