| CO2 activates orexin-containing neurons in mice. | |
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MedLine Citation:
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PMID: 19442935 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Accumulating evidence shows possible participation of orexin, a hypothalamic neuropeptide also known as hypocretin, in regulation of breathing. In this study we examined whether hypercapnic stimulation in vivo activates orexin-containing neurons in the hypothalamus. A hypercapnic gas mixture of 10% CO(2), 21% O(2), and residual N(2) or room air was inhaled to awake and freely moving mice for 3h. Their brains were examined by double immunohistochemical staining for orexin and c-Fos. We found that inhalation of CO(2) significantly increased c-Fos expression in the orexin-containing cells that were located in the perifornical region and the dorsomedial hypothalamus (15.8+/-1.6% for room air vs. 28.4+/-2.7% for CO(2), p=0.011) but not in the lateral hypothalamic area (12.3+/-1.2% for room air vs. 12.4+/-3.0% for CO(2)). Although current methodology cannot differentiate between direct effect of CO(2) on the orexin-containing neurons and indirect one through other neurons, this is the first report showing that inhalation of CO(2) did activate the orexin-containing neurons in vivo. |
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Authors:
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Jinko Sunanaga; Ben-Shiang Deng; Wei Zhang; Yuichi Kanmura; Tomoyuki Kuwaki |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-03-24 |
Journal Detail:
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Title: Respiratory physiology & neurobiology Volume: 166 ISSN: 1878-1519 ISO Abbreviation: Respir Physiol Neurobiol Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-05-15 Completed Date: 2009-08-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101140022 Medline TA: Respir Physiol Neurobiol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 184-6 Citation Subset: IM |
Affiliation:
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Department of Physiology, Kagoshima University Graduate School of Medical & Dental Sciences, Kagoshima 890-8544, Japan. jinko@m2.kufm.kagoshima-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / cytology*, drug effects Carbon Dioxide / pharmacology* Cell Count Intracellular Signaling Peptides and Proteins / metabolism* Male Mice Mice, Inbred C57BL Neurons / cytology, drug effects* Neuropeptides / metabolism* Proto-Oncogene Proteins c-fos / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Intracellular Signaling Peptides and Proteins; 0/Neuropeptides; 0/Proto-Oncogene Proteins c-fos; 0/orexins; 124-38-9/Carbon Dioxide |
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