Document Detail

CMV infection attenuates the disease course in a murine model of multiple sclerosis.
MedLine Citation:
PMID:  22393447     Owner:  NLM     Status:  MEDLINE    
Recent evidence in multiple sclerosis (MS) suggests that active CMV infection may result in more benign clinical disease. The goal of this pilot study was to determine whether underlying murine CMV (MCMV) infection affects the course of the Theiler's murine encephalitis virus (TMEV) induced murine model of MS. A group of eight TMEV-infected mice were co-infected with MCMV at 2 weeks prior to TMEV infection while a second group of TMEV-infected mice received MCMV two weeks post TMEV. We also used 2 control groups, where at the above time points MCMV was replaced with PBS. Outcome measures included (1) monthly monitoring of disability via rotarod for 8 months; (2) in vivo MRI for brain atrophy studies and (3) FACS analysis of brain infiltrating lymphocytes at 8 months post TMEV infection. Co-infection with MCMV influenced the disease course in mice infected prior to TMEV infection. In this group, rotarod detectable motor performance was significantly improved starting 3 months post-infection and beyond (p≤0.024). In addition, their brain atrophy was close to 30% reduced at 8 months, but this was only present as a trend due to low power (p = 0.19). A significant reduction in the proportion of brain infiltrating CD3+ cells was detected in this group (p = 0.026), while the proportion of CD45+ Mac1+ cells significantly increased (p = 0.003). There was also a strong trend for a reduced proportion of CD4+ cells (p = 0.17) while CD8 and B220+ cell proportion did not change. These findings support an immunomodulatory effect of MCMV infection in this MS model. Future studies in this co-infection model will provide insight into mechanisms which modulate the development of demyelination and may be utilized for the development of novel therapeutic strategies.
Istvan Pirko; Rhonda Cardin; Yi Chen; Anne K Lohrey; Diana M Lindquist; R Scott Dunn; Robert Zivadinov; Aaron J Johnson
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-02-29
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-03-06     Completed Date:  2012-07-23     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e32767     Citation Subset:  IM    
Department of Neurology, Mayo Clinic, Rochester, Minnesota, United States of America.
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MeSH Terms
Antigens, CD3 / biosynthesis
Antigens, CD45 / biosynthesis
Antigens, CD8 / biosynthesis
Brain / pathology
Cytomegalovirus Infections / complications,  therapy*,  virology
Demyelinating Diseases / virology
Disease Models, Animal
Encephalitis / virology
Flow Cytometry / methods
Immune System
Multiple Sclerosis / complications,  therapy,  virology*
Reproducibility of Results
Time Factors
Grant Support
Reg. No./Substance:
0/Antigens, CD3; 0/Antigens, CD8; EC, CD45

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